Mouse Anti-Fibronectin (Cell/Heparin2 Binding Region)
Protein G purified mouse monoclonal antibody to human Fibronectin (Cell/Heparin2 Binding region).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M013 |
| Isotype: | IgG1 |
| Immunogen: | Clone (M013) was generated from a proprietary antigen related to native human fibronectin from the A549 lung carcinoma cell line. |
| Immunogen Species: | Human |
| Species Reactivity: | Human |
| Gene Name: | FN1 |
| Molecular Weight: | 250 |
| Purification: | Protein G-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Clone M013 mouse monoclonal antibody detects a 250 kDa* protein on Western blots of native human A549, MeWo, and MDA-MB-231 cells, as well as human plasma fibronectin. The antibody does not detect the denatured form of fibronectin. The antibody also works for immunoprecipitation, immunohistochemistry, and immunocytochemistry, as well as binds fibronectin in live cells and unfixed extracellular matrix. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:50; IP 1:50. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Immunoprecipitation