Mouse Anti-Fibrillarin (Clone 38F3)

The antibody has been widely used as a convenient marker of nucleoli in many different species including human, rat, mouse, zebrafish, C. elegans, D. melanogaster, yeast and many others.

Mouse Anti-Fibrillarin (Clone 38F3)
Mouse Anti-Fibrillarin (Clone 38F3)
High magnification confocal image of HeLa cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin 1:100, green), colabeled with Anti-Vimentin red, 1:1000) showing the cytoplasmic intermediated filaments, and nuclear staining was done with DAPI (blue).
High magnification confocal image of HeLa cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin 1:100, green), colabeled with Anti-Vimentin red, 1:1000) showing the cytoplasmic intermediated filaments, and nuclear staining was done with DAPI (blue).
Western blot of HeLa cell lysate showing specific immunolabeling of the ~34 kDa fibrillarin protein.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~34 kDa fibrillarin protein.
Immunofluorescence of human neuroblastoma SH-SY5Y cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin 1:500, green), and nuclear staining was done with DAPI (blue) which makes the fibrillarin marker appear pale blue.
Immunofluorescence of human neuroblastoma SH-SY5Y cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin 1:500, green), and nuclear staining was done with DAPI (blue) which makes the fibrillarin marker appear pale blue.

Specifications

Physical State:
Liquid
Storage:
-20°C Short term, -80°C Long term
Buffer:
PBS, 50% glycerol, 5 mm sodium azide
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Product code: AMSFBLMC

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Unit priceFrom $154.00
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Product Overview

All three were made against recombinant full length human fibrillarin. Clone 38F3.

Host Species: Mouse
Clonality: Monoclonal
Clone: 38F3
Isotype: IgG1
Immunogen: Yeast nuclear preparation
Immunogen Species: Yeast
Species Reactivity: Bovine, Chicken, Drosophila, Human, Mouse, Pig, Rat
Gene Name: NOP1
Molecular Weight: 34 kDa
RRID: AB_2492093
Purification: Protein G-purified
Physical State: Liquid
Buffer: PBS, 50% glycerol, 5 mm sodium azide
Specificity: Specific for endogenous levels of the ~34 kDa Fibrillarin /Nop1p protein.
Application Notes: WB: 1:1000 IHC: 1:100-1:500 ICC: 1:100-1:500
Testing: Western blot on every lot.
Storage Conditions: -20°C Short term, -80°C Long term
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
Documents & Datasheets

Certificate of Analysis