Mouse Anti-E-Cadherin (Cytoplasmic)

Protein A purified mouse monoclonal antibody to human, mouse and rat E-Cadherin (Cytoplasmic).

Mouse Anti-E-Cadherin (Cytoplasmic)
Mouse Anti-E-Cadherin (Cytoplasmic)
Western blot image of human A431 cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). Blots were probed with anti-E-Cadherin (Cytoplasmic) and anti-N-Cadherin (Tyr-860)/E-Cadherin (Tyr-835) conserved site.
Western blot image of human A431 cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). Blots were probed with anti-E-Cadherin (Cytoplasmic) and anti-N-Cadherin (Tyr-860)/E-Cadherin (Tyr-835) conserved site.
Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Human 3D differentiated epidermis was incubated with roflumilast (Rof, 100nM) or vehicle (0.1% DMSO; control) for 30 min followed by the stimulation with TGFβ1 (10 ng/ml) for 48 h (collagen type I and E cadherin proteins
Human 3D differentiated epidermis was incubated with roflumilast (Rof, 100nM) or vehicle (0.1% DMSO; control) for 30 min followed by the stimulation with TGFβ1 (10 ng/ml) for 48 h (collagen type I and E cadherin proteins

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSECAG1-100UL

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Unit price$379.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M168
Isotype: IgG1
Immunogen: Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing residues in the cytoplasmic region. This sequence is highly conserved in human and rat E-cadherin.
Immunogen Species: Mouse
Species Reactivity: Human, Mouse, Rat
Gene Name: CDH1
Molecular Weight: 120
Purification: Protein A-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: This E-cadherin antibody detects a 120 kDa* protein in human A431 cells, and shows no cross-reactivity with VE-cadherin or N-cadherin.
Application Notes: Recommended starting dilutions: WB 1:1000; IHC 1:50; ICC 1:250; IP 1:100.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
  • Cancer research
Documents & Datasheets

Certificate of Analysis