Mouse Anti-CD47 (Extracellular Region)
Protein G purified mouse monoclonal antibody to human CD47 (Extracellular region) Antibody.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M054 |
| Isotype: | IgG1 |
| Immunogen: | Clone (M054) was generated from a recombinant protein that included the extracellular region of human CD47 protein. |
| Immunogen Species: | Human |
| Species Reactivity: | Human |
| Gene Name: | CD47 |
| Molecular Weight: | 45-55 |
| Purification: | Protein G-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Clone M054 mouse monoclonal antibody detects native human CD47, as well as aldehyde-fixed CD47. The antibody works for staining in immunocytochemistry, capture and detection in ELISA, native immunoprecipitation and western blot. The antibody detects a 45 to 55 kDa band in native western blots of A431, BEAS-2B, MCF7, NCI-H446, NCI-H28, and NCI-H2052 cell lines. |
| Application Notes: | Recommended starting dilutions: WB 1:500; ICC 1:200; IP 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Immunoprecipitation
- Cancer research