Mouse Anti-CD44 (Hyaluron Binding Region)
Protein G purified mouse monoclonal antibody to human CD44 (Hyaluron Binding Region).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M588 |
| Isotype: | IgG1 |
| Immunogen: | Clone M588 was generated from a proprietary antigen related to the hyaluron binding region of human CD44 from the MDA-MB-231 breast cancer cell line. |
| Immunogen Species: | Human |
| Species Reactivity: | Human |
| Gene Name: | CD44 |
| Molecular Weight: | 80-130 |
| Purification: | Protein G-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Clone M588 detects 80-130 kDa* bands matching the molecular mass of CD44 on Western blots of native MDA-MB-231, A431, and A549 cell lysates. The antibody also detects denatured CD44 but with lower affinity. In addition, Clone M588 binds the native form of a recombinant human CD44 protein that contains only the hyaluron binding region. The antibody can be used in multiple applications including western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well as for detecting CD44 in live, unfixed cells. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100; IP 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Immunoprecipitation
- Cancer research