Mouse Anti-Amyloid Beta Peptide (A-beta 40/42, Biotin)
Affinity purified mouse monoclonal antibody raised against Amyloid Beta Peptide (A-beta 40/42, Biotin).
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term), Protect from light
Buffer:
Learn more Lyophilized from PBS buffer, pH 7.4; contains no preservative
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | MOAB-2 |
| Immunogen: | Recombinant human amyloid beta protein 42 (Aβ42): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Rat |
| Purification: | IgG |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer, pH 7.4; contains no preservative |
| Specificity: | MOAB-2 detects preparations enriched in U-, O-, F-Aβ42, and U-Aβ40 by dot-blot, and is thus a pan-specific Aβ antibody. However, MOAB-2 is selective for the more neurotoxic Aβ42 compared to Aβ40. Indeed, MOAB-2 demonstrated a titration against antigen concentration, and detects Aβ40 at 2.5 pmol, but U-, O- and F-Aβb42 at antigen concentrations as low as ~ 0.1 pmol (Youmans. KL et al., 2012; PMID: 22423893). MOAB-2 does not detect APP (Amyloid Precursor Protein). Human, rat, other species not yet tested. By Dot Blot, MOAB-2 detected rat Aβ40 and human Aβ40, albeit with less affinity than for Aβ42 (Youmans KL et al., 2012). |
| Application Notes: | Recommended starting dilutions: WB 1:2000 – 1:5000; IHC 1:500 – 1:2000; ICC 1:100 – 1:500; IP 1:200 – 1:1000; ELISA 1:50 – 1:1000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term), Protect from light |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water to give a concentration of 1 mg/mL before use. Protect from light. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- ELISA
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Immunoprecipitation
- Neuroscience research