Mouse Anti-Alpha Internexin/ NF-66

Protein G purified mouse monoclonal antibody to human, mouse and rat Alpha Internexin/ NF-66.

Mouse Anti-Alpha Internexin/ NF-66
Mouse Anti-Alpha Internexin/ NF-66
Immunofluorescence of a section of rat cerebellum selectively labeling the axons of granule cells with alpha-internexin 1:5000, green) and colabeled with anti-calretinin (red).
Immunofluorescence of a section of rat cerebellum selectively labeling the axons of granule cells with alpha-internexin 1:5000, green) and colabeled with anti-calretinin (red).
Immunofluorescence of cultured rat CNS cells showing specific labeling of neuronal processes with Anti-alpha-internexin 1:250, red), and specific labeling of perikarya and dendrites with anti-microtubule associated protein 2 1:2,500,green).
Immunofluorescence of cultured rat CNS cells showing specific labeling of neuronal processes with Anti-alpha-internexin 1:250, red), and specific labeling of perikarya and dendrites with anti-microtubule associated protein 2 1:2,500,green).
Western blot of rat cortex lysate showing specific immunolabeling of the ~66 kDa alpha internexin protein.
Western blot of rat cortex lysate showing specific immunolabeling of the ~66 kDa alpha internexin protein.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: AMSINAG1

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Unit priceFrom $154.00
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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: 1D2
Isotype: IgG1
Immunogen: Full-length recombinant rat alpha-internexin, expressed in E. coli and purified.
Immunogen Species: Rat
Species Reactivity: Human, Mouse, Rat
Gene Name: INA
Molecular Weight: 66 kDa
RRID: AB_2492032
Purification: Protein G purified from culture supernatant
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~66 kDa alpha Internexin protein. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.
Application Notes: Recommended starting dilutions: WB 1:2000; WB Brain 1:2000; IHC 1:1000 – 1:5000; ICC 1:250 – 1:5000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis