Mouse Anti-Akt N-Terminus
Protein A purified mouse monoclonal antibody to human, mouse and rat Akt (N-terminal region).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M101 |
| Isotype: | IgG1 |
| Immunogen: | Clone M101 was generated from a recombinant protein containing residues in the N-terminal region of human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | AKT1 |
| Molecular Weight: | 60 |
| Purification: | Protein A-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects a 60 kDa* protein matching the apparent molecular mass of phoshorylated Akt on Western blots of A431 + calyculin A cell lysate. Similar results were seen in calyculin A treated human aortic endothelial and HeLa cells, rabbit spleen fibroblasts, and rat pituitary cells. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:250. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research