Goat Anti-Green Fluorescent Protein Tag (GFP)
Affinity purified goat polyclonal antibody raised against Green Fluorescent Protein Tag (GFP).
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgG.
| Host Species: | Goat |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Full-length recombinant AcGFP protein, expressed in E. coli and purified. |
| Immunogen Species: | Jellyfish |
| Molecular Weight: | 27 kDa |
| Purification: | Affinity-purified |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | Antibody recognizes GFP protein in GFP-transfected HEK293 cells, but not in non-transfected control cells. Specific for GFP, shows no cross-reactivity with mCherry. |
| Application Notes: | Recommended starting dilutions: WB 1:1000 – 1:5000; ICC 1:2000 – 1:5000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
