Chicken Anti-VGAT (Recombinant Chimeric)

Chicken monoclonal antibody to mouse and rat VGAT, purified by affinity chromatography.

Chicken Anti-VGAT (Recombinant Chimeric)
Chicken Anti-VGAT (Recombinant Chimeric)
Representative FFPE section of rat brain cerebellum showing VGAT staining. The sections were stained with anti-VGAT recombinant chicken monoclonal antibody using goat anti-chicken IgY FITC secondary antibody (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium. DAPI (blue) stains cell nuclei. Anti-VGAT stains the GABAergic terminals and neurons in the cerebellum. Magnification 100X.
Representative FFPE section of rat brain cerebellum showing VGAT staining. The sections were stained with anti-VGAT recombinant chicken monoclonal antibody using goat anti-chicken IgY FITC secondary antibody (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium. DAPI (blue) stains cell nuclei. Anti-VGAT stains the GABAergic terminals and neurons in the cerebellum. Magnification 100X.
Representative FFPE section of rat brain cerebellum showing VGAT staining. The sections were stained with anti-VGAT recombinant chicken monoclonal antibody using goat anti-chicken IgY FITC secondary antibody (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium. DAPI (blue) stains cell nuclei. Anti-VGAT stains the GABAergic terminals and neurons in the cerebellum. Magnification 400X.
Representative FFPE section of rat brain cerebellum showing VGAT staining. The sections were stained with anti-VGAT recombinant chicken monoclonal antibody using goat anti-chicken IgY FITC secondary antibody (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium. DAPI (blue) stains cell nuclei. Anti-VGAT stains the GABAergic terminals and neurons in the cerebellum. Magnification 400X.
The mouse mAb and chicken Mab were detected with anti-mouse-HRP and anti-chicken-HRP, respectively.
The mouse mAb and chicken Mab were detected with anti-mouse-HRP and anti-chicken-HRP, respectively.

Specifications

Physical State:
Liquid
Storage:
-20°C or below (long term), 2 – 8°C (short term)
Buffer:
PBS, 0.05% Sodium Azide pH 7.4

Release criteria

Protein Concentration:
1 mg/mL
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Product code: ACKVG1IYRC

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Unit priceFrom $144.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Recombinant Monoclonal
Clone: L118/80
Isotype: IgY
Immunogen: Recombinant fusion protein spanning residues 1-133 (cytoplasmic N-terminal region) of mouse VGAT (UniProt O35633), expressed in E. coli.
Immunogen Species: Mouse
Species Reactivity: Mouse, Rat
Gene Name: Slc32a1 Vgat Viaat
Molecular Weight: 50 kDa
Purification: Affinity-purified
Physical State: Liquid
Buffer: PBS, 0.05% Sodium Azide pH 7.4
Protein Concentration: 1 mg/mL
Specificity: No cross-reactivity has been reported.
Application Notes: Recommended starting dilutions: WB 1:1000; IHC 1:500.
Testing: Each lot is QC-tested by Western blot on rat whole-brain lysate for a band at the expected molecular weight.
Storage Conditions: -20°C or below (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening.
Shipping Conditions: Dry ice
Expiration: Stable for 24 months from receipt.

Product Applications

  • Western blot
  • ELISA
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis