Chicken Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1)

Chicken polyclonal antibody to bovine, human, mouse, pig and rat Ubiquitin C-terminal Hydrolase 1 (UCHL1), supplied as total IgY fraction.

Chicken Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1)
Chicken Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1)
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.
Immunostaining of E20 rat cortical neuron and glial culture stained with anti-UCHL1 antibody red, 1:500) and rabbit anti-vimentin antibody (green). The blue is DAPI staining nuclear DNA. The anti-UCHL1 stains strongly the cell body and dendrites of neurons, while anti-vimentin specifically stains intermediate filaments in fibroblasts and glia cells.
Immunostaining of E20 rat cortical neuron and glial culture stained with anti-UCHL1 antibody red, 1:500) and rabbit anti-vimentin antibody (green). The blue is DAPI staining nuclear DNA. The anti-UCHL1 stains strongly the cell body and dendrites of neurons, while anti-vimentin specifically stains intermediate filaments in fibroblasts and glia cells.
Immunostaining of mixed neuron/glial cultures stained with anti-UCHL1 antibody green, 1:500) and rabbit anti-GFAP antibody red, 1:1000). The blue stains nuclear DNA. The anti-UCHL1 stains strongly the cell body and dendrites of neurons, while anti-GFAP specifically labels astrocytes.
Immunostaining of mixed neuron/glial cultures stained with anti-UCHL1 antibody green, 1:500) and rabbit anti-GFAP antibody red, 1:1000). The blue stains nuclear DNA. The anti-UCHL1 stains strongly the cell body and dendrites of neurons, while anti-GFAP specifically labels astrocytes.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: ACKUC3IY

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Recombinant full-length human UCHL1 purified from E. coli.
Immunogen Species: Human
Species Reactivity: Bovine, Human, Mouse, Rat
Gene Name: UCHL1
Molecular Weight: 24 kDa
RRID: AB_2492281
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~24 kDa UCHL1 protein.
Application Notes: Recommended starting dilutions: WB 1:2500; WB Brain 1:2500; IHC 1:500 – 1:1000; ICC 1:500 – 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis