Chicken Anti-Tyrosine Hydroxylase (TYH)

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P24529) and human (P07101) gene products.

Chicken Anti-Tyrosine Hydroxylase (TYH)
Chicken Anti-Tyrosine Hydroxylase (TYH)
Immunostaining of paraformaldehyde-fixed (4%) free-floating sections (50 µm) of the posterior area of the brain (ventral tegmental area/substania nigra) of an adult mouse brain containing TH+ neurons. TH antibody) dilution @ 1:1500 with secondary detection of the chicken antibody with Alexa-647 anti-chicken IgY, 1:1000. (Image courtesy of Antonella Rios, Duke University.)
Immunostaining of paraformaldehyde-fixed (4%) free-floating sections (50 µm) of the posterior area of the brain (ventral tegmental area/substania nigra) of an adult mouse brain containing TH+ neurons. TH antibody) dilution @ 1:1500 with secondary detection of the chicken antibody with Alexa-647 anti-chicken IgY, 1:1000. (Image courtesy of Antonella Rios, Duke University.)
Immunostaining of paraformaldehyde-fixed (4%) free-floating sections (50 µm) of the posterior area of the brain (ventral tegmental area/substania nigra) of an adult mouse brain containing TH+ neurons. TH antibody) dilution @ 1:1500 with secondary detection of the chicken antibody with Alexa-647 anti-chicken IgY, 1:1000. (Image courtesy of Antonella Rios, Duke University.)
Immunostaining of paraformaldehyde-fixed (4%) free-floating sections (50 µm) of the posterior area of the brain (ventral tegmental area/substania nigra) of an adult mouse brain containing TH+ neurons. TH antibody) dilution @ 1:1500 with secondary detection of the chicken antibody with Alexa-647 anti-chicken IgY, 1:1000. (Image courtesy of Antonella Rios, Duke University.)
Western blot showing specific immunolabeling of tyrosine hydroxylase.
Western blot showing specific immunolabeling of tyrosine hydroxylase.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
In situ hybridization of Fgfr1c mRNA (green) and Klb mRNA (white), immunostaining for tyrosine hydroxylase (TH; 1:1000; blue), and the merge of the three. Two different magnifications are shown, with the boxed area in the top panels expanded in the bottom panels. Dotted lines outline the locus coeruleus. Image from publication CC-BY-4.0. PMID:36889282
In situ hybridization of Fgfr1c mRNA (green) and Klb mRNA (white), immunostaining for tyrosine hydroxylase (TH; 1:1000; blue), and the merge of the three. Two different magnifications are shown, with the boxed area in the top panels expanded in the bottom panels. Dotted lines outline the locus coeruleus. Image from publication CC-BY-4.0. PMID:36889282
Fluorescent Immunohistochemistry and RNAscope in situ hybridization was performed on samples from Phox2b‐Cre‐ZsGreen mice to visualize the presence if TH (1:2000) and Agtar1 mRNA respectively. (a, c, e, g) Immunoreactivity for TH and Agtar1 mRNA expression varied between neurons in the NG (profiles are dotted outlines 1 – 4). (b, d, f, h) Neither marker was detected in extraganglionic neurons in the vagus nerve (dotted outline). CC-BY-4.0. PMID:37403978
Fluorescent Immunohistochemistry and RNAscope in situ hybridization was performed on samples from Phox2b‐Cre‐ZsGreen mice to visualize the presence if TH (1:2000) and Agtar1 mRNA respectively. (a, c, e, g) Immunoreactivity for TH and Agtar1 mRNA expression varied between neurons in the NG (profiles are dotted outlines 1 – 4). (b, d, f, h) Neither marker was detected in extraganglionic neurons in the vagus nerve (dotted outline). CC-BY-4.0. PMID:37403978
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Chicken Anti-Tyrosine Hydroxylase (TYH)
Chicken Anti-Tyrosine Hydroxylase (TYH)
Immunostaining of noradrenergic neurons in the locus coeruleus of a C57BL/6J mouse labeling tyrosine hydroxylase (1:1000, green). Image kindly provided by Dr. Mihwa Choi, UT Southwestern Medical Center.
Immunostaining of noradrenergic neurons in the locus coeruleus of a C57BL/6J mouse labeling tyrosine hydroxylase (1:1000, green). Image kindly provided by Dr. Mihwa Choi, UT Southwestern Medical Center.
Immunostaining of adult mouse brain section fixed in 4% paraformaldehyde and preserved in cryoprotectant showing specific detection of tyrosine hydroxylase (1:500; green) in dopaminergic neurons of the substantia nigra. Nuclei stained with DAPI (blue). Background stain was attenuated with TrueVIEW. Image kindly provided by Ifeoluwa (Hiphy) Awogbindin of the Tremblay Lab, Division of Medical Sciences, University of Victoria, Canada.
Immunostaining of adult mouse brain section fixed in 4% paraformaldehyde and preserved in cryoprotectant showing specific detection of tyrosine hydroxylase (1:500; green) in dopaminergic neurons of the substantia nigra. Nuclei stained with DAPI (blue). Background stain was attenuated with TrueVIEW. Image kindly provided by Ifeoluwa (Hiphy) Awogbindin of the Tremblay Lab, Division of Medical Sciences, University of Victoria, Canada.

Specifications

Physical State:
Liquid
Storage:
2 – 8°C
Buffer:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative

Release criteria

Protein Concentration:
200 µg/mL
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Product code: ACKTH0IY

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Product Overview

Antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Synthetic peptides
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: TH TYH
Molecular Weight: 60 kDa
RRID: AB_10013440
Purification: Affinity-Purified IgY
Physical State: Liquid
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative
Protein Concentration: 200 µg/mL
Application Notes: Recommended starting dilutions: WB 1:1000 – 1:2000; IHC 1:1000 – 1:2000; ICC 1:1000 – 1:2000.
Testing: Both of the antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Storage Conditions: 2 – 8°C
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles.
Shipping Conditions: Dry ice
Expiration: Stable for 12 months from receipt.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis