Chicken Anti-Prostatic Acid Phosphatase (PAP)

Recombinant mouse PAP protein was expressed in bacteria. Antibodies against this protein were prepared by injecting purified recombinant PAP into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens.

Chicken Anti-Prostatic Acid Phosphatase (PAP)
Chicken Anti-Prostatic Acid Phosphatase (PAP)
Adult mouse DRG was fixed in 4% paraformaldehyde, cryostat-sectioned, and then stained for PAP immunoreactivity (1:500 dilution), showing immunoreactive material in primary sensory neurons. Photomicrograph by Dr. Mark Zilka, Univ. of North Carolina.
Adult mouse DRG was fixed in 4% paraformaldehyde, cryostat-sectioned, and then stained for PAP immunoreactivity (1:500 dilution), showing immunoreactive material in primary sensory neurons. Photomicrograph by Dr. Mark Zilka, Univ. of North Carolina.
Adult mouse spinal cord was fixed in 4% paraformaldehyde, paraffin-embedded and sectioned, and then sections were stained for PAP immunoreactivity (1:500 dilution). Adjacent sections were co-stained for IB4 and Calcitonin Gene-Related Protein (CGRP) (other sensory neuronal markers). Photomicrograph by Dr. Mark Zilka, University of North Carolina.
Adult mouse spinal cord was fixed in 4% paraformaldehyde, paraffin-embedded and sectioned, and then sections were stained for PAP immunoreactivity (1:500 dilution). Adjacent sections were co-stained for IB4 and Calcitonin Gene-Related Protein (CGRP) (other sensory neuronal markers). Photomicrograph by Dr. Mark Zilka, University of North Carolina.
Adult mouse spinal cord was fixed in 4% paraformaldehyde, paraffin-embedded and sectioned, and then sections were stained for PAP immunoreactivity (1:500 dilution). Immunoreactivity shown in Rexed Lamina 2 of the dorsal horn gray matter of the spinal cord. Photomicrograph by Dr. Mark Zilka, University of North Carolina.
Adult mouse spinal cord was fixed in 4% paraformaldehyde, paraffin-embedded and sectioned, and then sections were stained for PAP immunoreactivity (1:500 dilution). Immunoreactivity shown in Rexed Lamina 2 of the dorsal horn gray matter of the spinal cord. Photomicrograph by Dr. Mark Zilka, University of North Carolina.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent

Release criteria

Protein Concentration:
10 mg/mL
Learn more

Product code: ACKAC3IY-200UL

Custom/Bulk request
Unit price$301.00
Add to Quote

Lead time on this item is currently: 2-4 Weeks

Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426

Product Overview

These antibodies have been validated with human, mouse and rat tissues.

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Recombinant mouse PAP expressed in Escherichia coli.
Immunogen Species: Mouse
Species Reactivity: Human, Mouse, Rat
Gene Name: ACPP
Molecular Weight: 45 kDa
RRID: AB_2313557
Purification: Mixture of IgY fraction and affinity-purified antibodies
Physical State: Liquid
Buffer: Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent
Protein Concentration: 10 mg/mL
Application Notes: Recommended starting dilutions: WB 1:1000 – 1:2000; IHC 1:500 – 1:1000.
Testing: Antibodies were analyzed using immunohistochemistry with tissue sections through a 10%-formalin fixed adult mouse. Sections were examined for PAPpositive dorsal root ganglion sensory neurons. Secondary antibodies (fluorescein-labeled goat anti-chicken IgY, Aves Cat. #F-1004) were used at a concentration of 1:500.
Storage Conditions: -20°C
Shipping Conditions: Dry ice
Expiration: Stable for 12 months from receipt.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis