Chicken Anti-Prion Protein
Antibodies were prepared by injecting laying hens with purified recombinant human PRN protein.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent
Release criteria
Protein Concentration:
10 mg/mL
Lead time on this item is currently: 2-4 Weeks
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Product Overview
After a series of boosts, eggs were collected from hyperimmunized aniamsl and the IgY fraction prepared. Affinity purified antibodies were then isolated from the IgY fraction using a PRN protein column. Finally, affinity purified antibodies were mixed with the IgY fraction to make the final product. These antibodies have been validated with human, mouse and rat tissues.
| Host Species: | Chicken |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgY |
| Immunogen: | Reccombinant fragment of human prion. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | PRNP ALTPRP PRIP PRP |
| Molecular Weight: | 27 kDa |
| RRID: | AB_2313559 |
| Purification: | Mixture of IgY fraction and affinity-purified antibodies |
| Physical State: | Liquid |
| Buffer: | Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent |
| Protein Concentration: | 10 mg/mL |
| Application Notes: | Recommended starting dilutions: WB 1:2500 – 1:5000; IHC 1:1000 – 1:2000; ICC 1:1000 – 1:2000. |
| Testing: | Antibodies were analyzed by immunocytochemistry (1:1000 dilution) using cells transfected with the prion protein cDNA. |
| Storage Conditions: | -20°C |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research