Chicken Anti-Neurofilament NF-H

Chicken polyclonal antibody to all known mammal and chicken Neurofilament NF-H, supplied as total IgY fraction.

Chicken Anti-Neurofilament NF-H
Chicken Anti-Neurofilament NF-H
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H 1:25,000, red) in nuclei of neurons and specific labeling of GFAP 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H 1:25,000, red) in nuclei of neurons and specific labeling of GFAP 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody green, 1:25,000). The section is colabeled with anti-calbindin red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody green, 1:25,000). The section is colabeled with anti-calbindin red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody red, 1:25,000) and anti-NFL antibody green, 1:100).
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody red, 1:25,000) and anti-NFL antibody green, 1:100).
Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M 1:5000, red) and anti-Neurofilament-H 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M 1:5000, red) and anti-Neurofilament-H 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 1:500, red) and anti-Neurofilament H 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 1:500, red) and anti-Neurofilament H 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: ACKNFHIY

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Purified bovine NF-H
Immunogen Species: Bovine
Species Reactivity: Chicken
Gene Name: NEFH
Molecular Weight: 200 kDa
RRID: AB_2492160
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~200 kDa Neurofilament H protein.
Application Notes: Recommended starting dilutions: WB 1:50000; WB Brain 1:50000; IHC 1:5000 – 1:25,000; ICC 1:25,000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis