Chicken Anti-Microtubule Associated Protein 2 (MAP2)
Chicken polyclonal antibody to bovine, human, mouse and rat Microtubule Associated Protein 2 (MAP2), supplied as total IgY fraction.
Chicken Anti-Microtubule Associated Protein 2 (MAP2)Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2 red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 red, 1:5000) and Anti-FOX3 green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 1:1000, red) and anti-synapsin 1:1000, green).Costaining of the primary brain cells isolated from 18-day embryonic rats (E18). Neurons stained with α-MAP2 (green). Copurified astrocytes stained with α-GFAP (red). Image from publication CC-BY-4.0. PMID: 35652638Immunostaining of rat hippocampus showing specific immunolabeling of MAP2 red, 1:1000), Tau-1 (green), and nuclear DNA labeled with DAPI (blue). Photo courtesy of the Miriam Zamorano, University of Texas Health Science Center at Houston.Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP 1:1000, green) and anti-MAP2 (1:1000, red).Immunolabeling of mouse cortical cultures labeled with Anti-GFAP 1:1000, green) and anti-MAP2 (1:1000, red).Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody red, 1:500) and anti-MAP2 antibody green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.Immunofluorescence of rat cerebellum showing strong synaptic staining of VSNL1 (green) in the molecular layer (ML) and MAP2 red, 1:2500). The blue stain is nuclear DNA.Representative images of anti-MAP2 immunolabeled rat neurons at 5 DIV of T30 treatment. Image from publication CC-BY-4.0. PMID: 37454238(A-B). Primary hippocampal neurons were stained with MAP2 1:1000) as a negative control under basal conditions (CTRL) and with picrotoxin treatment (PTX). Image from publication CC-BY-4.0. PMID: 37654026Day 14 (D14) RGC-iNs stained with a DAPI, TAU, and MAP2 1:1000). Image from publication CC-BY-4.0. PMID: 37773257Representative 3D view images of paired helical filament (PHF)-tau-treated human brain organoid fractions. The organoids were labeled for MAP2 1:1000, green), NR3A (red), PSD95 (magenta), and DAPI (blue). White arrows show the positions colocalized between PSD95 and NR3A. The dotted circles show the magnified positions. Scale bar = 10 or 3 μm. Image from publication, CC-BY-4.0. PMID: 39396906
