Baby Rabbit Complement, IgG Depleted
Produced under controlled conditions from large pools of 21–28-day-old New Zealand White rabbits, then depleted of IgG to reduce heterophile antibody activity further. Useful in serum bactericidal and opsonophagocytic assays.Often used for serum bactericidal (rSBA) and opsonophagocytic (OPKA) assays, CDC potency assays and antibody-plus-complement T-cell depletion.
Specifications
Release criteria
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Species: | Rabbit |
|---|---|
| Product Types: | Complement |
| Depletion Process: | IgG Depleted |
| Sterility: | Sterile |
| Absorption: | Unabsorbed |
| Physical State: | Frozen Liquid |
| Hemolytic Titer: | ≥ 1:16 |
| Application Notes: | Suitable for functional antibody assays for vaccine evaluation (SBA - serum bactericidal assay; OPA/MOPA - opsonophagocytic killing assays), complement-dependent cytotoxicity (CDC) potency assays of therapeutic antibodies, and antibody-plus-complement depletion of cell populations - for example anti-Thy-1.2, anti-CD4 or anti-CD8 with complement at about 1:10 for 1 hour at 37°C to remove T cells from mouse splenocytes or bone marrow. Complement from 3-4-week-old rabbits has low heterophile antibody activity, so untreated cells are spared; titrate antibody and complement on your cells. Complement components are extremely heat-sensitive; thaw on ice or in a cold water bath (ideally under a stream of cold water, ~20°C), never in warm or hot water. Make single-use aliquots at first thaw and store at -70°C or below; avoid repeated freeze-thaw cycles, and discard rather than refreeze a thawed working aliquot. |
| Testing: | Hemolytic Titer: ≥1:16 Endotoxin: ≤ .0 EU/mL Residual IgG: <3.5% Sterility: Passed, 14 day sterility testing Mycoplasma: Negative Viral: Negative |
| Storage Conditions: | -70°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Poly bottle |
| Expiration: | 1 year from date of receipt when stored continuously at -70°C or below; avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Serum bactericidal assay
- Opsonophagocytic assay
- Complement-dependent cytotoxicity
- Complement-mediated cell depletion
- Hemolytic complement assay
- Vaccine development
Cited in Dittel 2010, J Vis Exp (complement depletion protocol)
Frequently Asked Questions
How does freezing and thawing affect complement activity?
Should I choose lyophilized or frozen liquid complement?
Can I use baby rabbit complement to deplete T cells or other cell populations?
What is complement activity (CH50 / hemolytic titer) and how is it tested?
Should I use human, rabbit, or guinea pig complement for my application?
Work backwards from the readout. Complement regulators are host-specific, so the complement source is part of the assay method, not a preference.
- Antibody-depleted human complement for the hSBA (serogroup B meningococcus and gonococcus; correlate hSBA ≥ 1:4). Neisseria recruits human factor H, which rabbit and guinea pig factor H cannot replace, and the donor antibodies in native human serum would kill the target on their own.
- Baby rabbit complement for the rSBA (serogroups A, C, W and Y; correlate rSBA ≥ 1:8) and for pneumococcal OPA/MOPA, which was standardized on it.
- Adult rabbit complement for complement-dependent cytotoxicity and HLA crossmatching, the classical cell-lysis source.
- Guinea pig complement where potency per volume is the point: CH50 titration, complement fixation and the vibriocidal assay.
When the model animal's own cascade is the readout, use matched-species complement. Mouse, rat, pig, canine, cynomolgus, goat and hamster complement are available on request through our custom quote form.
For what the research literature actually uses, by pathogen and assay, see which complement source for which assay.
Why am I seeing non-specific killing in my opsonophagocytic killing assay?
• Run the assay with a new aliquot of bacteria, in case the original was compromised in a way that made it easier to kill.
• Use a different bacterial strain to see if you can replicate the finding.
• Test a new HL60 cell preparation, preferably at a higher or lower passage number.
Show 5 more questions
How is virology testing for baby rabbit complement performed?
Can I order products from a single lot? Can I order products from several different lots?
Which pathogens have been studied in serum bactericidal assays using baby rabbit complement?
Is baby rabbit complement used in opsonophagocytic killing assays (OPA/OPKA)?
Is there a basic protocol for a serum bactericidal assay using rabbit complement?
Product References
- Human IgM inhibits the formation of titan-like cells in Cryptococcus neoformans
Nuria Trevijano-Contador et al. Human IgM inhibits the formation of titan-like cells in cryptococcus neoformans. Infect. Immun. 88, (2020).
- Physicochemical and biological similarity assessment of LBAL, a biosimilar to adalimumab reference product (Humira®)
Kwon, J.-C. et al. Physicochemical and biological similarity assessment of LBAL, a biosimilar to adalimumab reference product (Humira®). Animal Cells Syst. (Seoul). 1–13 (2021).
- Development of an Opsonophagocytic Killing Assay Using HL-60 Cells for Detection of Functional Antibodies against Streptococcus pyogenes
Salehi, S., Hohn, C. M., Penfound, T. A. & Dale, J. B. Development of an Opsonophagocytic Killing Assay Using HL-60 Cells for Detection of Functional Antibodies against Streptococcus pyogenes . mSphere 3, (2018).
- Functional Activity of Antibodies Directed towards Flagellin Proteins of Non-Typhoidal Salmonella
Ramachandran, G. et al. Functional activity of antibodies directed towards Flagellin proteins of non-typhoidal Salmonella. PLoS One 11, 151875 (2016).
- Cryptococcus neoformans Coinfection Dampens the TNF-α Response in HIV-1-Infected Human THP-1 Macrophages
Kalem, M. C., Humby, M. S., Wohlfert, E. A., Jacobs, A. & Panepinto, J. C. Cryptococcus neoformans Coinfection Dampens the TNF-α Response in HIV-1-Infected Human THP-1 Macrophages. mSphere 6, (2021).
- Investigating the candidacy of the serotype specific rhamnan polysaccharide based glycoconjugates to prevent disease caused by the dental pathogen Streptococcus …
St. Michael, F. et al. Investigating the candidacy of the serotype specific rhamnan polysaccharide based glycoconjugates to prevent disease caused by the dental pathogen Streptococcus mutans. Glycoconj. J. 35, 53–64 (2018).
- Rational design and evaluation of an artificial Escherichia coli K1 protein vaccine candidate based on the structure of OmpA
Gu, H. et al. Rational design and evaluation of an artificial Escherichia coli K1 protein vaccine candidate based on the structure of OmpA. Front. Cell. Infect. Microbiol. 8, 172 (2018).
- Immunization with Pseudomonas aeruginosa outer membrane vesicles stimulates protective immunity in mice
Zhang, X. et al. Immunization with Pseudomonas aeruginosa outer membrane vesicles stimulates protective immunity in mice. Vaccine 36, 1047–1054 (2018).
- Quality improvement of capsular polysaccharide in streptococcus pneumoniae by purification process optimization
Lee, C. et al. Quality Improvement of Capsular Polysaccharide in Streptococcus pneumoniae by Purification Process Optimization. Front. Bioeng. Biotechnol. 8, (2020).
- Validity of Enzyme-Linked Immunosorbent Assay (ELISA) as a correlate of protection of meningococcal C conjugated vaccine in adolescents with HIV infection
Daniela Vinhas, B. et al. Validity of Enzyme-Linked Immunosorbent Assay (ELISA) as a correlate of protection of meningococcal C conjugated vaccine in adolescents with HIV infection. J. Vaccines Immunol. 6, 001–007 (2020).
- Age-stratified analysis of serotype-specific baseline immunity against group B streptococcus
Choi, M. J. et al. Age-stratified analysis of serotype-specific baseline immunity against group B streptococcus. Hum. Vaccines Immunother. 16, 1338–1344 (2020).
- Performance of a Four-Antigen Staphylococcus aureus Vaccine in Preclinical Models of Invasive S. aureus Disease
Scully, I. L. et al. Performance of a four-antigen staphylococcus aureus vaccine in preclinical models of invasive S. aureus disease. Microorganisms 9, 1–13 (2021).
- A Structural Model for the Ligand Binding of Pneumococcal Serotype 3 Capsular Polysaccharide-Specific Protective Antibodies.
Ozdilek, A. et al. A Structural Model for the Ligand Binding of Pneumococcal Serotype 3 Capsular Polysaccharide-Specific Protective Antibodies. MBio (2021).
- Opsonophagocytic Antibodies of Intravenous Immunoglobulin Preparations against Seven Streptococcus agalactiae Serotypes
Lee, J. H., Kim, H. W., Cha, J. & Kim, K. H. Opsonophagocytic antibodies of intravenous immunoglobulin preparations against seven streptococcus agalactiae serotypes. Pediatr. Infect. Vaccine 28, 21–30 (2021).
- An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera
McGregor, R., Jones, S., Jeremy, R. M., Goldblatt, D. & Moreland, N. J. An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera. in Methods in Molecular Biology vol. 2136 323–335 (Humana Press Inc., 2020).
- Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines
Xu, H. et al. Expression of bordetella pertussis antigens fused to different vectors and their effectiveness as vaccines. Vaccines 9, (2021).
- Immunogenicity of PCV24, an expanded pneumococcal conjugate vaccine, in adult monkeys and protection in mice.
McGuinness, D. et al. Immunogenicity of PCV24, an expanded pneumococcal conjugate vaccine, in adult monkeys and protection in mice. Vaccine (2021).
Alternate Names
Young Rabbit Complement; 3-4 Week Rabbit Complement; 3-4 Week Old Rabbit Complement; Serum From Baby Rabbits; Pooled Baby Rabbit Serum; Pooled Baby Rabbit Complement
