Human Serum, Single Donor, Off the Clot, Heat Inactivated
Human off-the-clot serum from individual donor units is 0.2 µm filtered, aliquoted, heat-inactivated, and frozen.
Specifications
Physical State:
Frozen Liquid
Storage:
-70°C or below
Release criteria
Complement activity (CH50):
Negative
Lead time on this item is currently: 1-2 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
| Species: | Human |
|---|---|
| Product Types: | Serum |
| Pooling: | Single Donor |
| Donor Sex: | Mixed |
| Processing Method: | Heat Inactivated |
| Physical State: | Frozen Liquid |
| Application Notes: | Suitable as a negative matrix diluent. |
| Testing: | CH50: Negative Each donor unit was tested prior to pooling according to FDA guidelines for the detection of Hepatitis B Surface Antigen, Antibodies to HIV and HVC, HIV-1 RNA, HBV DNA, HCV RNA, WNV RNA, and Syphilis. Each donor has been tested according to FDA guidelines for T. Cruzi (Chagas). All units yielded NON-REACTIVE/NEGATIVE results for each test performed. All blood is collected in the United States of America from human donors in FDA licensed centers and tested with FDA approved test kits. No test method can provide total assurance that Hepatitis B Virus, Hepatitis C Virus, Human Immunodeficiency Virus or other infectious agents are absent. |
| Storage Conditions: | -70°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Cryovial, Poly bottle |
| Expiration: | 1 year from date of receipt when stored continuously at -70°C or below; avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Assay diluent & negative matrix
- Assay controls & calibrators
- Cell culture
- Vaccine development
- In vitro diagnostics
Documents & Datasheets
Certificate of Analysis
Frequently Asked Questions
How should I thaw frozen serum or plasma?
Put the bottle in a refrigerator at 2-8°C overnight, or in a 37°C water bath with the water kept below the cap, and swirl it gently as it thaws. Take it out as soon as the last ice has melted; never thaw above 37°C, on the bench or in a microwave. Then mix, aliquot what you will not use right away and return the aliquots to the label temperature. The full walkthrough with diagrams: how to thaw frozen serum.
Can I refreeze serum after it has thawed?
Aliquots can go back to the freezer once; the bulk bottle should not. Every freeze-thaw cycle concentrates salts, shifts pH and stresses the proteins, and complement activity drops with each one. Split the bottle into single-use tubes on the first thaw so each portion is only ever thawed once, or hold the thawed bottle at 2-8°C and use it within the window on the product page. See how to thaw frozen serum.
My thawed serum is cloudy or has particles in it. Is it still good?
Usually yes. Some haze or flocculent material after thawing is cryoprecipitate (fibrin, lipoprotein and calcium salts that came out of solution while frozen), and it does not change how the serum performs. Warm the bottle briefly to 37°C and swirl to redissolve most of it, or filter through a 0.45 µm filter if you need a clear product. A strong odor, a color change, or turbidity that keeps increasing at room temperature is contamination, not cryoprecipitate: do not use the bottle; contact us with the lot number. More in how to thaw frozen serum.
What is complement activity (CH50 / hemolytic titer) and how is it tested?
The hemolytic titer measures how well complement lyses antibody-sensitized sheep red blood cells; CH50 is the dilution of serum that lyses 50% of the cells. It is the release test for every lot of our complement products, and our heat-inactivated human products are CH50 tested to confirm that complement activity is absent.
What is the difference between pooled and single-donor products?
Pooled products combine material from multiple donors, which averages out donor-to-donor variation and gives large, uniform lots. Single-donor products come from one donor: their properties trace to that donor and can differ more between lots. Studies that need a single, well-characterized source, such as one complement lot carried through a clinical trial, use single-donor material.
What is heat-inactivated serum and when should I use it?
Heat-inactivated serum has been held at 56°C for 30 minutes to destroy complement activity. Use it when you need the other serum components without functional complement, for example as a cell culture supplement or an assay matrix where active complement could lyse cells or cause non-specific killing.
