Pel-Freez Blog
Read more about the latest updates, how Pel-Freez products support research, and get helpful practical tips in our blog.
-
Antibody Basics: Host, Target and Isotype ExplainedThe three attributes that define a research antibody, host species, target and isotype, and how to use them to pick the right antibody for...
-
Choosing a Host Species for Polyclonal and Monoclonal AntibodiesWhy host species matters for polyclonal and monoclonal antibodies: rabbit, goat, sheep, chicken and rodent hosts compared on affinity and cross-reactivity.
-
Antibodies in IHC, Western Blot and ELISA: Applications and TipsHow antibodies are used in immunohistochemistry, Western blotting and ELISA, with practical selection and optimization tips for reproducible results.
-
Using Acetone Powders for Antibody Pre-adsorptionHow tissue acetone powders remove cross-reactive antibodies before IHC, Western blot or ELISA, with a step-by-step pre-adsorption protocol.
-
Don’t waste your valuable sera samples!The high analytical error rate in immunoassays is well documented, estimated to range from 0.4% to 4.0%. These errors generate misleading or even false...
-
Tips for Antibody PurificationProtein A, G and L resins cover most antibodies. Where they fall short, and when NHS-activated or multimodal beads are the better route.
-
The Future of Antibodies in NeuroscienceAntibodies are the workhorse reagent of cellular and molecular neuroscience. A short look at how they are used today and where reproducibility and validation...
