Rabbit Anti-Tyrosine Hydroxylase Antibody
Antigen affinity-purified rabbit polyclonal antibody to tyrosine hydroxylase; detects mammalian and at least some non-mammalian TH. For Western blot, IHC/IF and ICC.
Specifications
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma. |
| Immunogen Species: | Rat |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | TH |
| Molecular Weight: | 60 kDa |
| RRID: | AB_2492276 |
| Purification: | Antigen affinity-purified from pooled serum |
| Processing Method: | Affinity Purified |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50% Glycerol |
| Specificity: | Recognizes endogenous ~60 kDa tyrosine hydroxylase protein. |
| Application Notes: | Recommended starting dilution: WB, IF, IHC 1:1000 |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C Short term, -80°C Long term |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Cold Pack |
| Packaging: | Microtube |
| Expiration: | 1 year from date of receipt when stored at -20°C (short term) or -80°C (long term); aliquot and avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Immunoprecipitation
- Neuroscience research
Documents & Datasheets
Datasheet
Safety Datasheet
Certificate of Analysis
Frequently Asked Questions
What dilution should I use for your antibodies in western blot, ELISA or formalin-fixed paraffin-embedded tissue?
Should I store antibodies at -20°C or -80°C, and should I aliquot them on delivery?
Publications citing this product (12)
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A novel rat model of body first subtype of Parkinsons disease
Where the paper cites Pel-Freez
…for 1 hour at room temperature to block nonspecific binding sites and then incubated in primary antibody (rabbit anti- tyrosine hydroxylase, Pel Freez Biologicals #P40101-150, 1:250 in blocking solution) for 72 hours at 4oC on a shaker. Sections were washed in between incubation steps with 6 x 3min PBS-Tx. Sections were incubated in diluted secondary antibody (biotinylated donkey anti-rabbit Ig
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High molecular weight insoluble parkin in the substantia nigra of patients with idiopathic Parkinson’s disease
Where the paper cites Pel-Freez
…1 h at ambient temperature. As for the immunoblots, the antibodies against parkin C-Terminal region (monoclonal parkin clone [PRK8], 1:1,000 and polyclonal parkin (cell signaling cat#2132, 1:1,000)), TH (Pel-Freez Biolo- gicalsCat# P40101-150, 1:1,000),αsyn(SYN1,BDBiosciencesCat# 610787, 1:1,000), MJFR1 (Abcam Cat# ab138501 [MJFR1], 1:1,000) and αsyn phosphorylatedatserine129(αsynP129)(AbcamCat#ab168381[MJF-R13…
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Integration of cross-species multi-omics with in vivo experimental validation identifies Parkinson's disease therapeutic targets and novel risk factors within endolysosomal pathway subnetworks
Where the paper cites Pel-Freez
…times for 5 min each using 1× PBST. Samples were placed in blocking buffer (1% bovine serum albumen in PBST) for 1 h at room temperature and then incubated with anti-Tyrosine Hydroxylase (1:500, P40101 PelFreez) for 3 days at 4 °C. After the primary was removed, samples were washed 3 times with 1× PBST for 5 min each, then incubated in secondary (1:500 Alexa Fluor anti-Rabbit 555 (A-21428) and 1:1000 DAPI) for 1…
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Loss of PINK1 causes age-dependent mitochondrial trafficking deficits in nigrostriatal dopaminergic neurons via aberrant p38 MAPK activation
Where the paper cites Pel-Freez
…an investigator blinded to the genotype. ARTICLE IN PRESS ARTICLE IN PRESS Antibodies and Reagents The antibodies and reagents used in the study include: Anti-Tyrosine Hydroxylase (rabbit, 1:5000 for IF, Pel-Freez, P40101-0), Anti-Tyrosine Hydroxylase (mouse, 1:500 for IF, Millipore, MAB5280), Anti-Kinesin 5B heavy chain (mouse, 1:500 for WB, Millipore, MAB1614), Anti- Dynein medium chain (mouse, 1:1000 for WB,…
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Miro1 mutations disrupt cellular calcium homeostasis via dysregulation of mitochondria-ER-contact-sites, rendering iPSC-derived neurons more susceptible to lipid peroxidation
Where the paper cites Pel-Freez
…Peprotech, 450-10-50UG). Neuronal cultures were assessed by immunostaining with antibodies against Mikrotubuli-associated protein 2 (MAP2; Abcam ab5392, dilution 1:10000) and Tyrosin Hydroxylase (TH; Pel-Freez P40101, dilution 1:1000). 2.2 Live cell imaging of mitochondrial calcium Neurons were cultivated in 8 well chamber slides (Ibidi, 80806) at 100,000 cells per well. Then, neurons were transfected with 0.5…
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Targeting reverse electron transport preserves mitochondrial function and prevents dopaminergic neurodegeneration in Parkinson’s disease
Where the paper cites Pel-Freez
…then incubated at 4 °C overnight with a rabbit polyclonal primary antibody against TH, GFAP or IBA-1 in KPBS containing 0.4% triton X-100 (TH, Pel Freez #P40101- 150, 1:1000; GFAP, Proteintech #16,825–1-AP 1:500; and Iba-1, Novus biologicals Cat#NBP2-19,019, 1:500). After five to six rinses in KPBS, the sections were treated for two hours in a biotinylated secondary antibody (PK- 6200, 1:1000,
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TWIK-1 plays distinct roles in spinal and peripheral sensory circuits controlling mechanical sensitivity and neuropathic hypersensitivity
Where the paper cites Pel-Freez
…No. 019-19741), goat anti-CGRP (1:1000, Abcam, Cat. No. ab36001), rabbit anti-CSF1R (1:100, Abcam, Cat. No. ab183316), chicken anti-NF200 (1:500, Aves labs, Cat. No. AB_2313552), rabbit anti-TH (1:1000, Pel-Freez, Cat. No. P40101- 150), and rabbit anti-mCherry (1:1000, Abcam, Cat. No. ab167453). The secondary antibodies used were Alexa Fluor-conjugated donkey anti-rabbit (1:500, Life Technologies, Cat. No.…
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Activation of the Carotid Body by Kappa Opioid Receptors Mitigates Fentanyl-Induced Respiratory Depression
Where the paper cites Pel-Freez
…ry neurons by µ opioid receptors and suggest that KOR agonists might counteract the central depressive effects of opioids on breathing. … The primary antibodies used were rabbit anti-TH antibody (Pel-Freez Biologicals; #P40101-0; dilution 1:1000), mouse anti-TH antibody (Sigma, #T1299, dilution 1:1000), guinea pig anti…
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Hemoglobin as a pseudoperoxidase and drug target for oxidative stress-related diseases
Where the paper cites Pel-Freez
…anti-Hbβ (1:200, Abcam, ab214049), mouse anti-Hbβ (1:200, Santacruz, sc-21757), mouse anti-MAOB (1:200, Santacruz, sc-515354), mouse anti-8-OhdG (1:500, Santacruz, sc-393871), rabbit anti-TH (1:500, Pelfreez, p40101), and mouse anti-TH (1:200, Sigma, T2928). The following secondary antibodies from Jackson were diluted (1:500) in blocking solution: donkey-anti-chicken 405, donkey-anti-chicken 647,…
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Impulsive-compulsive behaviours and striatal neuroactivity in mildly parkinsonian rats under D2/3 agonist and L-DOPA treatment
Where the paper cites Pel-Freez
…analysis. For quantifying the extent of striatal dopaminergic denervation and counting of pS6 + cells, bright-field immunohistochemistry was performed using a primary antibody against TH (rabbit anti-TH, Pel-Freez P40101, 1:1000) and phosphorylated pS6, respectively (monoclonal rabbit anti Ser235/236-phospho-S6, Cell Signaling #2211, 1:200). Immunocomplexes were revealed using biotinylated secondary antibodies…
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Microglia depletion reduces neurodegeneration and remodels extracellular matrix in a mouse Parkinson’s disease model triggered by α-synuclein overexpression
Where the paper cites Pel-Freez
…30 min to eliminate endogenous oxidase. The samples were then blocked with 5% normal goat serum (ZSGB-Bio, Cat#: ZLI-9021, Lot#: 190320) for 1 h at room temperature, then incubated with rabbit anti-TH (Pel-Freez, Cat#: P40101, Lot#: aj0520P, 1:1000) or rabbit anti-NeuN (EMD Millipore Corp, Cat#: ABN78, Lot#: 3692639, 1:500) overnight. On the second day, the brain slices were incubated with a secondary antibody…
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Molecularly distinct striatonigral neuron subtypes differentially regulate locomotion
Where the paper cites Pel-Freez
…Antifade Mountant, Life technology). Images were taken using a laser scanning confocal microscope LSM 780 (Zeiss). The primary antibodies used for immunostaining included rabbit monoclonal anti-TH (Pel-Freez Biologicals, P40101; dilution 1:1000), mouse monoclonal anti-TH (ImmunoStar, 22941; dilution 1:1000), chicken polyclonal anti-TH (Aves Labs, TYH; dilution 1:500), rabbit anti-Mu-opioid receptor (MOR)…
Product References
- Fyn knockdown prevents levodopa-induced dyskinesia in a mouse model of Parkinson’s disease
Bordone, M. P. et al. Fyn knockdown prevents levodopa-induced dyskinesia in a mouse model of Parkinson’s disease. eneuro ENEURO.0559-20.2021 (2021).
- An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
Gantner, C. W., Cota-Coronado, A., Thompson, L. H. & Parish, C. L. An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions. STAR Protoc. 1, 100065 (2020).
- β‑Lapachone ameliorates L‑DOPA‑induced dyskinesia in a 6‑OHDA‑induced mouse model of Parkinson's disease
Ryu, Y. K. et al. β-Lapachone ameliorates L-DOPA-induced dyskinesia in a 6-OHDA-induced mouse model of Parkinson’s disease. Mol. Med. Rep. 23, (2021).
- Splenic artery loops: Potential splenic plexus stimulation sites for neuroimmunomodulatory‐based anti‐inflammatory therapy?
Cleypool, C. G. J., Lotgerink Bruinenberg, D., Roeling, T., Irwin, E. & Bleys, R. L. A. W. Splenic artery loops: Potential splenic plexus stimulation sites for neuroimmunomodulatory-based anti-inflammatory therapy? Clin. Anat. 34, 371–380 (2021).
- Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing
Park, H., Shin, J., Choi, H., Cho, B. & Kim, J. Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing. Cells 9, (2020).
- Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson's disease
Jarras, H. et al. Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson’s disease. J. Neuroendocrinol. 32, (2020).
Alternate Names
TH; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase
