Chicken Anti-V5

Affinity purified chicken polyclonal antibody raised against V5.

Chicken Anti-V5
Chicken Anti-V5
Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.
Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.
Photo courtesy of Thomas Coate.
Photo courtesy of Thomas Coate.
Western blot of HEK293 cells transfected with V5-tagged CASR (lane 1) or mock transfected cells (lane 2) using chicken α-V5 antibody showing specific immunolabeling at the expected molecular weight
Western blot of HEK293 cells transfected with V5-tagged CASR (lane 1) or mock transfected cells (lane 2) using chicken α-V5 antibody showing specific immunolabeling at the expected molecular weight
DAPI (blue) was used for DNA staining. The image was kindly provided by Arlene Hirano, University of California, Los Angeles.
DAPI (blue) was used for DNA staining. The image was kindly provided by Arlene Hirano, University of California, Los Angeles.

Specifications

Physical State:
Liquid
Storage:
2 – 8°C
Buffer:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative

Release criteria

Protein Concentration:
0.2mg/mL
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Product code: ACKV51IY

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Unit priceFrom $144.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Isotype IgY.

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein.
RRID: AB_2910559
Purification: Affinity purified
Physical State: Liquid
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative
Protein Concentration: 0.2mg/mL
Application Notes: Recommended starting dilutions: WB 1:500 – 1:1000; ICC 1:100 – 1:500.
Testing: Each lot is checked for a single band at the expected molecular weight by Western blot.
Storage Conditions: 2 – 8°C
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles.
Shipping Conditions: Dry ice
Expiration: Stable for 12 months from receipt.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis