Rat Anti-APC (central region)
Protein G purified rat monoclonal antibody to human, mouse and rat APC (Central region).
Specifications
Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
Learn more PBS, 0.05% sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rat |
|---|---|
| Clonality: | Monoclonal |
| Clone: | KT44 |
| Isotype: | IgG2a |
| Immunogen: | Clone KT44 was generated from a recombinant protein containing residues from the central region mouse APC. This sequence is highly conserved in human and rat APC, and has low homology to APC2. |
| Immunogen Species: | Mouse |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | APC |
| Molecular Weight: | 300 |
| Purification: | Protein G-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 0.05% sodium azide |
| Specificity: | Detects full length APC at 300 kDa* and proteolytic fragments of APC at lower molecular weights. The antibody detects APC or its fragments in SW480, CaCO2, DLD-1, and Hct116 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules. |
| Application Notes: | Recommended starting dilutions: WB 1:250; ICC 1:50. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C or -80°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research