Rabbit Anti-Tyrosine Hydroxylase

Rabbit polyclonal antibody to all mammalian Tyrosine Hydroxylase, antigen affinity purified from pooled serum.

Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0.1 to 0.3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.
Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0.1 to 0.3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.
Immunostaining of E17 rat midbrain mixed neuronal cultures showing TH positive neurons green, 1:500) and MAP2 red, 1:2000). Before labeling, cells were fixed with 4% PFA in PBS and permeabilized for 1 hour 0.3 % Triton X. Image courtesy of Aurélie de Rus Jacquet, laboratory of Dr. Jean-Christophe Rochet, Purdue University.
Immunostaining of E17 rat midbrain mixed neuronal cultures showing TH positive neurons green, 1:500) and MAP2 red, 1:2000). Before labeling, cells were fixed with 4% PFA in PBS and permeabilized for 1 hour 0.3 % Triton X. Image courtesy of Aurélie de Rus Jacquet, laboratory of Dr. Jean-Christophe Rochet, Purdue University.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
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Product code: ARBTH0V3

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Unit priceFrom $164.00
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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.
Immunogen Species: Rat
Species Reactivity: Human, Mouse, Rat
Gene Name: TH
Molecular Weight: 60 kDa
RRID: AB_2492276
Purification: Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns
Physical State: Liquid
Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Specificity: Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein.
Application Notes: WB: 1:1000 WB Brain: 1:1000 IHC: 1:1000 ICC: 1:500-1:1000
Testing: Western blot on every lot.
Storage Conditions: -20°C
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • ELISA
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis