Rabbit Anti-TrkB Phospho Ser478/479
Affinity purified rabbit polyclonal antibody raised against TrkB Phospho Ser478/479.
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized from PBS, pH 7.2-7.6, with 0.1% trehalose
Lead time on this item is currently: 2-4 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
Isotype IgG.
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immunogen, SNDDDSA[pS]PLHHIS. |
| Immunogen Species: | Human |
| Species Reactivity: | Chicken, Human, Mouse, Rat |
| Molecular Weight: | 120 kDa |
| Purification: | Affinity-purified |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS, pH 7.2-7.6, with 0.1% trehalose |
| Specificity: | Antibody is specific for TrkB serine phosphorylation at amino acid 478/479 (rodents/human). Lambda-phosphatase treatment obliterates positive staining. Human TrkB (pS478). Antibody has been shown to be specific for TrkB phosphorylated on serine 478 by phospho-peptide absorption dot blots, and on cell lysates from cell lines induced with retinoic acid and BDNF. Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only, demonstrating that the phosphorylated TrkB receptor is being detected. Additional non-specific bands at lower molecular weight may be observed in lysates and homogenates with the antibody and these bands have not been characterized. |
| Application Notes: | Recommended starting dilutions: WB 0.5-2 µg/mL; IHC 1-5 µg/mL. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunohistochemistry
- Neuroscience research