Rabbit Anti-Spectrin, Alpha II
Affinity purified rabbit polyclonal antibody raised against Spectrin.
Specifications
Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized with sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgG.
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Raised against a mix of five recombinant constructs containing the entire C-terminal region of human alpha-II spectrin (residues 676-2,400). |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse |
| Molecular Weight: | 240 kDa |
| Purification: | Whole serum (unpurified antiserum) |
| Physical State: | Liquid |
| Buffer: | Lyophilized with sodium azide |
| Specificity: | Reacts with a 240 kDa band by Western blot on mouse sciatic nerve extract. Minor bands below may be seen and this likely represents in vivo proteolytic fragments of alpha-II spectrin. It has also been used successfully for immunocytochemistry. |
| Application Notes: | Recommended starting dilutions: WB 1:5000 – 1:10000; ICC 1:500 – 1:1000. |
| Storage Conditions: | -20°C or -80°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)