Rabbit Anti-P-WAVE1 (Tyr125)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat WAVE1 (Tyr-125).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-WAVE (Tyr-125) peptide (conjugated to KLH) matching residues around Tyr-125 in human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in the conserved site in WAVE2 (Tyr-124) and WAVE3 (Tyr-125). |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | WASF1 |
| Molecular Weight: | 80 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to phospho-WAVE (Tyr-150) and unphosphorylated WAVE (Tyr-125) peptides before affinity purification using phospho-WAVE (Tyr-125) peptide. In western blots, the antibody detects an 80 kDa* band matching phosphorylated WAVE in human SYF cSrc transformed cells and mouse macrophages treated with pervanadate, but is not observed in control cells. These bands can be specifically blocked with phospho-WAVE (Tyr-125) peptide (WX1775), but not phospho-WAVE (Tyr-150) peptide (WX1825). |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:200. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research