Rabbit Anti-P-Sox2 (Thr128)
Antigen affinity purified rabbit polyclonal antibody to chicken, human, mouse, rat and Xenopus Sox2 (Thr-128).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-Sox2 (Thr-128) synthetic peptide (conjugated to carrier) matching residues around Thr-128 in mouse Sox2. This site is identical in human Sox2 (Thr-126), and has some homology with the conserved site in mouse Sox1 (Ser-136) and Sox3 (Ser-154). |
| Immunogen Species: | Mouse |
| Species Reactivity: | Chicken, Frog, Human, Mouse, Rat |
| Gene Name: | SOX2 |
| Molecular Weight: | 34 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross adsorbed to unphosphorylated Sox2 (Thr-128) peptide before affinity purification using phospho-Sox2 (Thr-128) peptide. This antibody detects a 34 kDa* protein on Western blots of mouse F9 stem cells. This reactivity is not observed after lambda phosphatase dephosphorylation of Sox2. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research