Rabbit Anti-Phospho-NMDA NR2B Subunit (Tyr1472) Antibody
Affinity-purified rabbit polyclonal antibody. Tested by Western blot on rat tissue.
Specifications
Physical State:
Liquid
Storage:
-20°C Short term, -80°C Long term
Buffer:
Learn more 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50% Glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide from residues around Tyr1472 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH). |
| Immunogen Species: | Rat |
| Species Reactivity: | Mouse, Rat |
| Gene Name: | GRIN2B |
| Molecular Weight: | 180 kDa |
| RRID: | AB_2492182 |
| Purification: | Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50% Glycerol |
| Specificity: | Recognizes endogenous ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1472. Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Application Notes: | Recommended starting dilution: WB 1:1000 |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C Short term, -80°C Long term |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | 1 year from date of receipt when stored at -20°C (short term) or -80°C (long term); aliquot and avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Western blot
- Neuroscience research
Documents & Datasheets
Datasheet
Certificate of Analysis
Frequently Asked Questions
What dilution should I use for your antibodies in western blot, ELISA or formalin-fixed paraffin-embedded tissue?
A typical starting dilution for antibodies in immunoassays is 1:1000. Consider adjusting the dilution depending on your experimental conditions, to increase or decrease the signal-to-noise ratio, etc.
Should I store antibodies at -20°C or -80°C, and should I aliquot them on delivery?
Either is fine, but avoid repeated freeze-thaw cycles. Our antibodies contain glycerol, so they do not freeze at -20°C but do at -80°C. If you will use the antibody within a few weeks, store it at -20°C. For long-term storage, split it into single-use aliquots and store at -80°C.
Alternate Names
GRIN2B; NR2B; NMDAR2B; GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NR3
