Rabbit Anti-P-EB3 (Ser176)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat EB3 (Ser-176).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-EB3 (Ser-176) synthetic peptide (conjugated to KLH) matching residues around serine 176 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3. This site is conserved in EB2 but the neighboring residues are not well conserved. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | MAPRE3 |
| Molecular Weight: | 32 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-absorbed to unphospho-EB3 (Ser-176), before affinity purification using phospho-EB3 (Ser-176) peptide (without carrier). The antibody detects a 32 kDa* protein matching the apparent molecular mass of phosphorylated EB3 on Western blots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment. |
| Application Notes: | Recommended starting dilution: WB 1:500. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research