Rabbit Anti-P-c-Src (Tyr215)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat c-Src (Tyr-215).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | C-Src (Tyr-215) synthetic peptide (conjugated to KLH) matching residues around tyrosine 215 of human c-Src. This sequence has homology to the conserved site in other Src family members, such as hck, fyn, csk, and yes. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | SRC |
| Molecular Weight: | 60 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Src (Tyr-215) peptide (without carrier). The antibody detects a 60 kDa* protein on Western blots of mouse SYF cells transformed with c-Src and human A431 cells that have been treated with pervanadate. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research