Rabbit Anti-P-AIFM1 (Ser116)

Antigen affinity purified rabbit polyclonal antibody to human AIFM1 (Ser-116).

Rabbit Anti-P-AIFM1 (Ser116)
Rabbit Anti-P-AIFM1 (Ser116)
Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).
Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).
Western blotting shows KEAP1-PGAM5-AIFM1 pathway protein level regulation by ALT. SKOV-3 cells were treated with different concentrations (0, 16, 32 and 64 μM) of ALT for 24 h. Specifically AIFM1, P-AIFM1(Ser-116), KEAP1, Nrf2, and PGAM5 levels were detected. B-Actin used as a control. Image from publication CC-BY-4.0. PMID: 37712005
Western blotting shows KEAP1-PGAM5-AIFM1 pathway protein level regulation by ALT. SKOV-3 cells were treated with different concentrations (0, 16, 32 and 64 μM) of ALT for 24 h. Specifically AIFM1, P-AIFM1(Ser-116), KEAP1, Nrf2, and PGAM5 levels were detected. B-Actin used as a control. Image from publication CC-BY-4.0. PMID: 37712005
Western blot analysis of protein expression of ASIC1, KEAP1, PGAM5, and AIFM1 phosphorylated at Ser116 (1:1000) in NPCs after treatment for 30 min with 20 nM Psalmotoxin 1 (PcTx1) followed by 24 h of treatment with H2O (control) or 100 μM TBHP. Image from publication CC-BY-4.0. PMID: 38540291
Western blot analysis of protein expression of ASIC1, KEAP1, PGAM5, and AIFM1 phosphorylated at Ser116 (1:1000) in NPCs after treatment for 30 min with 20 nM Psalmotoxin 1 (PcTx1) followed by 24 h of treatment with H2O (control) or 100 μM TBHP. Image from publication CC-BY-4.0. PMID: 38540291

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: ARBAI4-100UL

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Unit price$348.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Phospho-AIFM1 (Ser-116) synthetic peptide (conjugated to carrier) matching residues around Ser-116 in human AIFM1. This site is conserved in rat and mouse AIFM1, but the residues around the site are not well conserved. The site is not found in AIFM2 and AIFM3.
Immunogen Species: Human
Species Reactivity: Human
Gene Name: AIFM1
Molecular Weight: 57/62/66
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: The antibody was cross adsorbed to unphosphorylated AIFM1 (Ser-116) peptide before affinity purification using phospho-AIFM1 (Ser-116) peptide. This antibody antibody detects a triplet at 66, 62, and 57 kDa* protein on Western blots of human Jurkat cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylated AIFM1.
Application Notes: Recommended starting dilution: WB 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Cancer research
Documents & Datasheets

Certificate of Analysis