Rabbit Anti-Olig2

Protein A purified rabbit polyclonal antibody to human, mouse and rat Olig2.

Rabbit Anti-Olig2
Rabbit Anti-Olig2
Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).
Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~32 kDa Olig2 protein.
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~32 kDa Olig2 protein.
An illustration differentiating mouse and human oligodendrocytes in the process of glial humanization of a mouse brain model used to study HIV-1 infection. Immunoflourescence of paraffin embedded chimeric mouse brain 5-µm thick tissue section immunolabeling oligodendrocytes with anti-OLIG2 antibody 1:500, green) and a human specific anti-nuclei antibody(clone 3E1.3). The blue stain is DAPI. Images courtesy Larisa Poluektova, University of Nebraska Medical Center.
An illustration differentiating mouse and human oligodendrocytes in the process of glial humanization of a mouse brain model used to study HIV-1 infection. Immunoflourescence of paraffin embedded chimeric mouse brain 5-µm thick tissue section immunolabeling oligodendrocytes with anti-OLIG2 antibody 1:500, green) and a human specific anti-nuclei antibody(clone 3E1.3). The blue stain is DAPI. Images courtesy Larisa Poluektova, University of Nebraska Medical Center.
Immunostaining of Olig 2 in 3-month-old mouse brain Olig 2: Red; Nuclear: Blue. Image courtesy of Mengmeng Jin, Rutgers University.
Immunostaining of Olig 2 in 3-month-old mouse brain Olig 2: Red; Nuclear: Blue. Image courtesy of Mengmeng Jin, Rutgers University.
Representative images of mouse NPC differentiation into Nkx6.1- and Olig2-positive neural progenitors after 3 days induction. Image from publication CC-BY-4.0. PMID:38664376
Representative images of mouse NPC differentiation into Nkx6.1- and Olig2-positive neural progenitors after 3 days induction. Image from publication CC-BY-4.0. PMID:38664376

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
100 mM Glycine, 200 mM Tris, pH 8.0
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Product code: ARBO20

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Unit priceFrom $164.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Recombinant mouse Olig2.
Immunogen Species: Mouse
Species Reactivity: Human, Mouse, Rat
Gene Name: OLIG2
Molecular Weight: 27 kDa
RRID: AB_2492193
Purification: Protein A purified
Physical State: Liquid
Buffer: 100 mM Glycine, 200 mM Tris, pH 8.0
Specificity: Recognizes endogenous ~32 kDa Olig2 protein.
Application Notes: Recommended starting dilutions: WB 1:3000; WB Brain 1:3000; IHC 1:200 – 1:1000; ICC 1:1000; IP 1:500 – 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis