Rabbit Anti-NSE (Neuron Specific Enolase)

Rabbit polyclonal antibody to bovine, chicken, human, mouse and rat NSE (Neuron specific enolase), supplied as neat serum.

Rabbit Anti-NSE (Neuron specific enolase)
Rabbit Anti-NSE (Neuron specific enolase)
Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody red, 1:500) and anti-GFAP antibody green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.
Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody red, 1:500) and anti-GFAP antibody green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.
Immunolabeling of HEK 293 cells labeled with anti-neuron specific enolase antibody red, 1:500) and anti-UCHL1 antibody green, 1:500).
Immunolabeling of HEK 293 cells labeled with anti-neuron specific enolase antibody red, 1:500) and anti-UCHL1 antibody green, 1:500).
Western blot of rat cortical lysate showing specific immunolabeling of the ~47 kDa NSE protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~47 kDa NSE protein.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
Neat serum
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Product code: ARBNSEV1

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Full-length recombinant human NSE, expressed in E. coli and purified.
Immunogen Species: Human
Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Gene Name: ENO2
Molecular Weight: 47 kDa
RRID: AB_2492190
Purification: Neat Serum
Physical State: Liquid
Buffer: Neat serum
Specificity: Recognizes endogenous ~47 kDa NSE protein.
Application Notes: Recommended starting dilutions: WB 1:2000; WB Brain 1:2000; IHC 1:500; ICC 1:500.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis