Rabbit Anti-NMDA NR2B Subunit Antibody, Lyophilized
Affinity-purified rabbit polyclonal antibody. Recognizes human, mouse and rat forms of the NR2B subunit of NMDAR. No reactivity towards the NR2A and NR2C subunits. Immunolabeling is blocked by pre-adsorption with the immunizing fusion protein.
Specifications
Physical State:
Lyophilized Powder
Storage:
-20°C Short term, -80°C Long term
Buffer:
Learn more Neat serum
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Fusion protein from the C-terminal region of the NR2B subunit of the rat NMDA receptor. |
| Immunogen Species: | Rat |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | GRIN2B |
| Molecular Weight: | 180 kDa |
| RRID: | AB_2492177 |
| Purification: | Neat Pooled Serum |
| Physical State: | Lyophilized Powder |
| Buffer: | Neat serum |
| Specificity: | Recognizes endogenous ~180 kDa NR2B subunit of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits. |
| Application Notes: | Recommended starting dilution: WB 1:1000; IHC 1:1000 to 1:2000; IP 3 µL per 200 µg lysate |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C Short term, -80°C Long term |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. |
| Shipping Conditions: | Dry ice |
| Expiration: | 1 year from date of receipt when stored at -20°C (short term) or -80°C (long term); aliquot and avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunoprecipitation
- Antibody pre-adsorption
- Neuroscience research
Documents & Datasheets
Datasheet
Certificate of Analysis
Frequently Asked Questions
What dilution should I use for your antibodies in western blot, ELISA or formalin-fixed paraffin-embedded tissue?
A typical starting dilution for antibodies in immunoassays is 1:1000. Consider adjusting the dilution depending on your experimental conditions, to increase or decrease the signal-to-noise ratio, etc.
Should I store antibodies at -20°C or -80°C, and should I aliquot them on delivery?
Either is fine, but avoid repeated freeze-thaw cycles. Our antibodies contain glycerol, so they do not freeze at -20°C but do at -80°C. If you will use the antibody within a few weeks, store it at -20°C. For long-term storage, split it into single-use aliquots and store at -80°C.
Alternate Names
GRIN2B; NR2B; NMDAR2B; GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NR3
