Rabbit Anti-NMDA NR2B Subunit
Rabbit polyclonal antibody to human, mouse and rat NMDA NR2B Subunit, antigen affinity purified from pooled serum.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Fusion protein from the C-terminal region of the NR2B subunit of the rat NMDA receptor. |
| Immunogen Species: | Rat |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | Grin2b |
| Molecular Weight: | 180 kDa |
| RRID: | AB_2492176 |
| Purification: | Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol |
| Specificity: | Specific for endogenous levels of the ~180 kDa NR2B subunit of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; WB Brain 1:1000; IHC 1:1000; IP 3 µl per 200 µg lysate. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunoprecipitation
- Neuroscience research