Rabbit Anti-NMDA NR2A Subunit Antibody, Lyophilized
Affinity-purified rabbit polyclonal antibody. Recognizes human, mouse and rat forms of the NR2B subunit of NMDAR. No reactivity towards the NR2A and NR2C subunits. Immunolabeling is blocked by pre-adsorption with the immunizing fusion protein.
Specifications
Physical State:
Lyophilized Powder
Storage:
-20°C Short term, -80°C Long term
Buffer:
Learn more Neat serum
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Fusion protein from the C-terminal region of the NR2A subunit of the rat NMDA receptor. |
| Immunogen Species: | Rat |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | GRIN2A |
| Molecular Weight: | 180 kDa |
| RRID: | AB_2492172 |
| Purification: | Neat Pooled Serum |
| Physical State: | Lyophilized Powder |
| Buffer: | Neat serum |
| Specificity: | Recognizes endogenous ~180 kDa NR2A subunit of the NMDA receptor. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. |
| Application Notes: | Recommended starting dilution: WB 1:1000; IHC 1:1000 to 1:2000; IP 3 µL per 200 µg lysate |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C Short term, -80°C Long term |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. |
| Shipping Conditions: | Dry ice |
| Expiration: | 1 year from date of receipt when stored at -20°C (short term) or -80°C (long term); aliquot and avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunoprecipitation
- Antibody pre-adsorption
- Neuroscience research
Documents & Datasheets
Datasheet
Certificate of Analysis
Frequently Asked Questions
What dilution should I use for your antibodies in western blot, ELISA or formalin-fixed paraffin-embedded tissue?
A typical starting dilution for antibodies in immunoassays is 1:1000. Consider adjusting the dilution depending on your experimental conditions, to increase or decrease the signal-to-noise ratio, etc.
Should I store antibodies at -20°C or -80°C, and should I aliquot them on delivery?
Either is fine, but avoid repeated freeze-thaw cycles. Our antibodies contain glycerol, so they do not freeze at -20°C but do at -80°C. If you will use the antibody within a few weeks, store it at -20°C. For long-term storage, split it into single-use aliquots and store at -80°C.
Alternate Names
GRIN2A; NR2A; NMDAR2A; GluN2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; hNR2A
