Rabbit Anti-MuRF1 C-Terminus

Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat MuRF1 (C-terminal region).

Rabbit Anti-MuRF1 C-Terminus
Rabbit Anti-MuRF1 C-Terminus
Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).
Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).
Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.
Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.
Western blot analysis of MuRF1, Atrogin-1, FoxO3a, p-AKT (Ser473), and total AKT in the soleus muscle of control, HU, and HU+TUDCA mice. Image from publication CC-BY-4.0. PMID: 37525907
Western blot analysis of MuRF1, Atrogin-1, FoxO3a, p-AKT (Ser473), and total AKT in the soleus muscle of control, HU, and HU+TUDCA mice. Image from publication CC-BY-4.0. PMID: 37525907
Western blotting analysis of the expression of Atrogin-1 (1:1000) and MuRF1 (1:1000) proteins in the soleus muscles after HS. Image from publication CC-BY-4.0. PMID: 37344807
Western blotting analysis of the expression of Atrogin-1 (1:1000) and MuRF1 (1:1000) proteins in the soleus muscles after HS. Image from publication CC-BY-4.0. PMID: 37344807
Western blot showing MuRF-1 (1:500) and atrogin-1, 1:500) levels in C2C12 myoblasts differentiated for 4–5 days were incubated with 200 μM UDCA for 72 h. GAPDH was used as a loading control. Image from publication CC-BY-4.0. PMID: 37237400
Western blot showing MuRF-1 (1:500) and atrogin-1, 1:500) levels in C2C12 myoblasts differentiated for 4–5 days were incubated with 200 μM UDCA for 72 h. GAPDH was used as a loading control. Image from publication CC-BY-4.0. PMID: 37237400
Markers for muscle degeneration, Myostatin and MuRF-1 (1:1000), were significantly less expressed in CTMP-KO gastrocnemius muscle compared to WT mice. Image from publication CC-BY-4.0. PMID: 36652767
Markers for muscle degeneration, Myostatin and MuRF-1 (1:1000), were significantly less expressed in CTMP-KO gastrocnemius muscle compared to WT mice. Image from publication CC-BY-4.0. PMID: 36652767
The individual and combined effect of quercetin (Q) and low dose (LL) or high dose (HL) of leucine on relative protein expression of p-FoxO1/FoxO1, Atrogin-1, and MuRF1 in the gastrocnemius muscle in BALB/c mice exposed to cisplatin (CDDP). Image from publication CC-BY-4.0. PMID: 37699022
The individual and combined effect of quercetin (Q) and low dose (LL) or high dose (HL) of leucine on relative protein expression of p-FoxO1/FoxO1, Atrogin-1, and MuRF1 in the gastrocnemius muscle in BALB/c mice exposed to cisplatin (CDDP). Image from publication CC-BY-4.0. PMID: 37699022

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: ARBT63-100UL

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Unit price$348.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: MuRF1 (C-terminal) synthetic peptide (conjugated to KLH) matching residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55).
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: TRIM63
Molecular Weight: 30/38
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects 38 and 30 kDa* proteins matching the apparent molecular mass of MuRF1 isoforms on Western blots of mouse C2C12 cells, and detects a 38 kDa band in mouse heart and muscle tissue.
Application Notes: Recommended starting dilutions: WB 1:1000; ICC 1:300.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis