Mouse Anti-Ubiquilin 2
Affinity purified mouse monoclonal antibody raised against Ubiquilin 2.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
Isotype IgG1.
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | 6H9 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human ubiquilin 2 expressed and purified from E. coli. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse |
| Molecular Weight: | ~66 kDa in human Hela cells and 68 kDa in rodent 3T3 cells |
| Purification: | IgG |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all tranduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in cells transduced with human wild type or mutant ubiquilin 2. Small proteins which run at 50 kDa in these cells are the fragments of ubiquilin 2. Note, ubiquilin 2 runs at ~66 kDa in human Hela cells and 68 kDa in rodent 3T3 cells. The antibody has also been used successfully for immunocytochemistry. |
| Application Notes: | Recommended starting dilutions: WB 1:1000 – 1:2000; ICC 1:500 – 1:1000. |
| Storage Conditions: | -20°C |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Neuroscience research