Mouse Anti-Plexin A1 (Sema Domain)

Antigen affinity purified mouse monoclonal antibody to human, mouse and rat Plexin A1 (Sema Domain).

Mouse Anti-Plexin A1 (Sema Domain)
Mouse Anti-Plexin A1 (Sema Domain)
Western blots showing mouse recombinant Plexin-A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin-A1 at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 at 1:250 (lane 3) and 1:1000 (lane 4).
Western blots showing mouse recombinant Plexin-A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin-A1 at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 at 1:250 (lane 3) and 1:1000 (lane 4).
Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1. The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1. The antibody was detected using goat anti-mouse DyLight® 594.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSPADG1-100UL

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Unit price$379.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M535
Isotype: IgG1
Immunogen: Clone M535 was generated from a Plexin-A1 synthetic peptide (conjugated to carrier protein) matching a region within the N-terminal half of the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: PLXNA1
Molecular Weight: 220
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 220 kDa* protein matching the apparent molecular mass of Plexin-A1 on Western blots of neonatal mouse brain, and detects a recombinant mouse Plexin A1 protein containing the extracellular region.
Application Notes: Recommended starting dilutions: WB 1:500; IHC 1:100; ICC 1:100; IP 1:50.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis