Mouse Anti-PKCθ N-Terminus
Protein A purified mouse monoclonal antibody to human, mouse and rat PKCθ (N-terminal region).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M217 |
| Isotype: | IgG2a |
| Immunogen: | Clone (M217) was generated from a recombinant mouse PKCθ protein that included residues in the N-terminal region. This sequence is conserved in human and rat PKCθ, and has low homology to other PKC family members. |
| Immunogen Species: | Mouse |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | PRKCQ |
| Molecular Weight: | 79 |
| Purification: | Protein A-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects a 79 kDa* protein matching the molecular mass of PKCθ on Western blots of human Jurkat cell lysates. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research