Mouse Anti-Peripherin

Mouse monoclonal antibody to mouse and rat Peripherin, supplied as concentrated tissue culture supernatant.

Immunostaining of cultured newborn rat neurons and glia showing peripherin green, 1:200) and neurofilament L (red). The nuclear DNA is stained blue.
Immunostaining of cultured newborn rat neurons and glia showing peripherin green, 1:200) and neurofilament L (red). The nuclear DNA is stained blue.
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~57 kDa peripherin protein.
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~57 kDa peripherin protein.
Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin red, 1:500) and anti-vimentin green, 1:10,000). The blue is Hoechst staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.
Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin red, 1:500) and anti-vimentin green, 1:10,000). The blue is Hoechst staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
Concentrated tissue culture supernatant + 10 mM NaN3
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Product code: AMSPRPG1

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Unit priceFrom $164.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: 7C5
Isotype: IgG1
Immunogen: Full-length recombinant rat peripherin, expressed in E. coli and purified.
Immunogen Species: Rat
Species Reactivity: Mouse, Rat
Gene Name: PRPH
Molecular Weight: 57 kDa
RRID: AB_2492207
Purification: Concentrated tissue culture supernatant
Physical State: Liquid
Buffer: Concentrated tissue culture supernatant + 10 mM NaN3
Specificity: Recognizes endogenous ~57 kDa peripherin protein. This antibody performs well on aldehyde fixed tissues.
Application Notes: WB: 1:1000 WB Brain: 1:1000 IHC: 1:200 ICC: 1:200-1:500
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • ELISA
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis