Mouse Anti-P-Stat5 (Tyr694)

Protein A purified mouse monoclonal antibody to human, mouse and rat Stat5 (Tyr-694).

Mouse Anti-P-Stat5 (Tyr694)
Mouse Anti-P-Stat5 (Tyr694)
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 or Stat5 (Tyr-694) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 or Stat5 (Tyr-694) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSS51G1-100UL

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Unit price$379.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M148
Isotype: IgG1
Immunogen: Clone (M148) was generated from a synthetic peptide (conjugated to KLH) matching residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: STAT5A
Molecular Weight: 92
Purification: Protein A-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 92 kDa* band matching Stat5A on Western blots of human A431 cells treated with EGF, but does not detect this band in control cells.
Application Notes: Recommended starting dilutions: WB 1:1000; ICC 1:100.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Cancer research
Documents & Datasheets

Certificate of Analysis