Mouse Anti-P-CREB (Ser133)
Protein A purified mouse monoclonal antibody to chicken, human, mouse and rat CREB (Ser-133).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M415 |
| Isotype: | IgG1 |
| Immunogen: | Clone M415 was generated from phospho-CREB (Ser-133) synthetic peptide (conjugated to carrier protein) matching residues around Ser-133 in human CREB. The sequence is conserved in rat, mouse, chicken, and zebrafish CREB. The site is also conserved in CREM (Ser-136) and ATF1 (Ser-63). |
| Immunogen Species: | Human |
| Species Reactivity: | Chicken, Human, Mouse, Rat |
| Gene Name: | CREB1 |
| Molecular Weight: | 43 |
| Purification: | Protein A-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects a 43 kDa* protein matching the molecular mass of CREB on Western blots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, as well as A431 treated with EGF and PC12 treated with NGF or Forskolin. This reactivity is not observed after lambda phosphatase treatment. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research