Mouse Anti-Mitofilin
Protein G purified mouse monoclonal antibody to human Mitofilin.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M027 |
| Isotype: | IgG1 |
| Immunogen: | Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line. |
| Immunogen Species: | Human |
| Species Reactivity: | Human |
| Gene Name: | IMMT |
| Molecular Weight: | 90 |
| Purification: | Protein G-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Clone M027 detects a 90 kDa* band matching the molecular mass of mitofilin on Western blots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues. In addition, mass spectrometry analysis of immunoprecipitates using MM0271 in human A431 cell lysates confirms this antibody only detects Mitofilin protein. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100; IP 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Immunoprecipitation