Mouse Anti-MeCP2

Protein G purified mouse monoclonal antibody to human, mouse and rat MeCP2.

Mouse Anti-MeCP2
Mouse Anti-MeCP2
Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 1:1000, red) in nuclei of neurons and specific labeling of GFAP 1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 1:1000, red) in nuclei of neurons and specific labeling of GFAP 1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.
Western blot of mouse whole brain lysate showing specific immunolabeling of the MeCP2 protein at ~75 kDa.
Western blot of mouse whole brain lysate showing specific immunolabeling of the MeCP2 protein at ~75 kDa.
Immunostaining of a section of rat hippocampus showing specific nuclear immunolabeling of MeCP2 red, 1:1000) and FOX3 (green). The blue is DAPI staining of nuclear DNA.
Immunostaining of a section of rat hippocampus showing specific nuclear immunolabeling of MeCP2 red, 1:1000) and FOX3 (green). The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 1:500, red), colabeled with anti-GFAP 1:5000, green), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.
Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 1:500, red), colabeled with anti-GFAP 1:5000, green), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 50% glycerol, 5 mM sodium azide
Learn more

Product code: AMSME2GB

Custom/Bulk request
Unit priceFrom $164.00
Add to Quote

Lead time on this item is currently: 2-4 Weeks

Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426

Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: 5H12
Isotype: IgG2b
Immunogen: Full length human recombinant MeCP2.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: MECP2
Molecular Weight: 75 kDa
RRID: AB_2632401
Purification: Protein G-purified from culture supernatant
Physical State: Liquid
Buffer: PBS, 50% glycerol, 5 mM sodium azide
Specificity: Recognizes endogenous MeCP2 protein. Due to usually high charge, the protein runs at ~75 kDa in SDS-PAGE of mouse brain lysates.
Application Notes: Recommended starting dilutions: WB 1:2000; WB Brain 1:2000; IHC 1:500 – 1:5000; ICC 1:1000 – 1:5000.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis