Mouse Anti-Integrin β1 (Extracellular Region)
Protein G purified mouse monoclonal antibody to human Integrin β1 (Extracellular region).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M004 |
| Isotype: | IgG1 |
| Immunogen: | Clone M004 was generated from a proprietary antigen related to the extracellular region of human integrin β1 in complex with integrin α expressed in the A431 epidermoid carcinoma cell line. |
| Immunogen Species: | Human |
| Species Reactivity: | Human |
| Gene Name: | ITGB1 |
| Molecular Weight: | 120-150 |
| Purification: | Protein G-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Clone M004 detects 120-150 kDa* bands matching Integrin β1 on Western blots of native human A431, A549, MDA-MB-231, and LNCaP cell lysates. The antibody also detects native recombinant human Integrin α1/β1, α2/β1, and α3/β1 complexes, but does not detect recombinant human Integrin α6/β4 or αV/β3 complexes. The antibody does not detect the denatured form of Integrin β1. Clone M004 can be used in western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well for detecting live, unfixed cells. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100; IP 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Immunoprecipitation
- Cancer research