Mouse Anti-HSP60 N-Terminus
Protein A purified mouse monoclonal antibody to human, mouse and rat Hsp60 (N-terminal region).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M438 |
| Isotype: | IgG1 |
| Immunogen: | Clone M438 was generated from a recombinant protein matching residues in the N-terminal region of human Hsp60. This sequence has high homology to similar regions in rat and mouse Hsp60. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | HSPD1 |
| Molecular Weight: | 60 |
| Purification: | Protein A-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects a 60 kDa* protein matching the apparent molecular mass of Hsp60 on Western blots of human Jurkat and rat A7r5 cells. In immunocytochemistry, anti-Hsp60 specifically stains mitochondria in paraformaldehyde fixed and NP-40 permeabilized cells. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)