Mouse Anti-FOX3 (NeuN)

Protein G purified mouse monoclonal antibody to bovine, human, mouse and rat FOX3 (NeuN).

Mouse Anti-FOX3 (NeuN)
Mouse Anti-FOX3 (NeuN)
Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.
Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3 green, 1:1000) and Anti-MAP2 red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3 green, 1:1000) and Anti-MAP2 red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP 1:1000, green) and nuclear staining was done with DAPI (blue).
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP 1:1000, green) and nuclear staining was done with DAPI (blue).
Cell Line: human-derived iPSC cerebral organoids. Neurofilament-H (green), NeuN (purple), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Cell Line: human-derived iPSC cerebral organoids. Neurofilament-H (green), NeuN (purple), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Cell Line: human-derived iPSC cerebral organoids. MAP2 (pink), NeuN (yellow), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Cell Line: human-derived iPSC cerebral organoids. MAP2 (pink), NeuN (yellow), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L 1:2000, green) and labeling of FOX3/NeuN 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L 1:2000, green) and labeling of FOX3/NeuN 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat hippocampus showing specific labeling of UCHL1 1:5000, green) in cell bodies and dendrites of neurons, and specific labeling of FOX3 (red). The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat hippocampus showing specific labeling of UCHL1 1:5000, green) in cell bodies and dendrites of neurons, and specific labeling of FOX3 (red). The blue is DAPI staining of nuclear DNA.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 50% glycerol, 5 mm sodium azide
Learn more

Product code: AMSFO3GB

Custom/Bulk request
Unit priceFrom $164.00
Add to Quote

Lead time on this item is currently: 2-4 Weeks

Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426

Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: 1B7
Isotype: IgG2b
Immunogen: Full-length recombinant protein taken from the N-terminal region of human FOX3, expressed in E. coli and purified.
Immunogen Species: Human
Species Reactivity: Bovine, Human, Mouse, Rat
Gene Name: RBFOX3
Molecular Weight: 46/48 kDa
RRID: AB_2492096
Purification: Protein G-purified from culture supernatant
Physical State: Liquid
Buffer: PBS, 50% glycerol, 5 mm sodium azide
Specificity: Recognizes endogenous ~46/48 kDa FOX3 protein doublet.
Application Notes: Recommended starting dilutions: WB 1:2000; WB Brain 1:2000; IHC 1:200 – 1:1000; ICC 1:200 – 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis