Mouse Anti-c-FOS

Protein G purified mouse monoclonal antibody to bovine, human, mouse and rat c-FOS.

Mouse Anti-c-FOS
Mouse Anti-c-FOS
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS 1:1000, green) while Anti-Vimentin 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS 1:1000, green) while Anti-Vimentin 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Immunostaining of mouse brain labeling c-Fos protein in green 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
Immunostaining of mouse brain labeling c-Fos protein in green 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
Representative images of CA1 region of mouse hippocampus. White arrows indicate cFos+ cells), yellow arrows indicate 53BP1 + cells. Nuclei stained with DAPI (blue). Right: zoomed images of the white boxes. Image from publication CC-BY-4.0. PMID: 36732588
Representative images of CA1 region of mouse hippocampus. White arrows indicate cFos+ cells), yellow arrows indicate 53BP1 + cells. Nuclei stained with DAPI (blue). Right: zoomed images of the white boxes. Image from publication CC-BY-4.0. PMID: 36732588
Immunolabeling of a section of rat brain that specifically labels c-FOS protein green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
Immunolabeling of a section of rat brain that specifically labels c-FOS protein green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
(A, B) confocal microscope images showing PV, WFA, and c-Fos) staining after vehicle or ABC treatment in the prelimbic PFC after vehicle or ABC 31 days later. White arrows indicate triple-labelled cells. Note faint staining of PNNs after ABC treatment. Image from publication CC-BY-4.0. PMID: 37855072.
(A, B) confocal microscope images showing PV, WFA, and c-Fos) staining after vehicle or ABC treatment in the prelimbic PFC after vehicle or ABC 31 days later. White arrows indicate triple-labelled cells. Note faint staining of PNNs after ABC treatment. Image from publication CC-BY-4.0. PMID: 37855072.
Immunofluorescence of young male and female wild type (WT) and Alzheimer's Disease (AD) mouse brain labelling nucleus-specific c-Fos + cells in the DMH, VMH, ARH, TuN, and LH regions. Image from publication CC-BY-4.0. PMID: 39272160
Immunofluorescence of young male and female wild type (WT) and Alzheimer's Disease (AD) mouse brain labelling nucleus-specific c-Fos + cells in the DMH, VMH, ARH, TuN, and LH regions. Image from publication CC-BY-4.0. PMID: 39272160

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 50% glycerol, 5 mM sodium azide
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Product code: AMSFOSG1

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Unit priceFrom $164.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: 2H2
Isotype: IgG1
Immunogen: Full-length recombinant human c-FOS, expressed in E. coli and purified.
Immunogen Species: Human
Species Reactivity: Bovine, Human, Mouse, Rat
Gene Name: FOS
Molecular Weight: 50 kDa
RRID: AB_2632380
Purification: Protein G-purified from culture supernatant
Physical State: Liquid
Buffer: PBS, 50% glycerol, 5 mM sodium azide
Specificity: Recognizes endogenous ~50 kDa c-FOS protein.
Application Notes: Recommended starting dilutions: WB 1:2000; IHC 1:1000; ICC 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Cancer research
Documents & Datasheets

Certificate of Analysis