Human Anti-Centromere Protein

Human polyclonal antibody to Centromere proteins, supplied as antisera.

Human Anti-Centromere Protein
Human Anti-Centromere Protein
A microscopy image of a cell from BJ hTERT cell line treated with aphidicolin to induce genomic instability. Immunolabeling of the cell positively labelling CREST/Centromere Protein (red, 1:500, ) and labelling DNA with DAPI. The anaphase cell has a segregation error which lacks centromeric staining unlike the mass of properly segregating chromosomes. Image kindly provided by Nadeem Shaikh and Sarah McClelland from Barts Cancer Institute, Queen Mary University of London.
A microscopy image of a cell from BJ hTERT cell line treated with aphidicolin to induce genomic instability. Immunolabeling of the cell positively labelling CREST/Centromere Protein (red, 1:500, ) and labelling DNA with DAPI. The anaphase cell has a segregation error which lacks centromeric staining unlike the mass of properly segregating chromosomes. Image kindly provided by Nadeem Shaikh and Sarah McClelland from Barts Cancer Institute, Queen Mary University of London.
Confocal image of kinetochore-microtubule attachments in mouse control (CON) and IMQ treated oocytes where kinetochores are dyed purple by CREST antibody, microtubules are stained green by the anti-tubulin antibody. Chromosomes are dyed blue by Hoechst 33342.Image from publication CC-BY-4.0. PMID: 35589679
Confocal image of kinetochore-microtubule attachments in mouse control (CON) and IMQ treated oocytes where kinetochores are dyed purple by CREST antibody, microtubules are stained green by the anti-tubulin antibody. Chromosomes are dyed blue by Hoechst 33342.Image from publication CC-BY-4.0. PMID: 35589679
Immunofluorescence showing SCP3 (green) and the co-localization of VCL (red) and CREST-labeling centromeres (blue) on squashed mouse spermatocytes. Image from publication CC-BY-4.0. PMID: 35805097
Immunofluorescence showing SCP3 (green) and the co-localization of VCL (red) and CREST-labeling centromeres (blue) on squashed mouse spermatocytes. Image from publication CC-BY-4.0. PMID: 35805097
Immunofluorescence of control and nivalenol (NIV) treated mouse oocytes where α-tubulin is labeled green, centromeres are labeled red and DNA is dyed blue by Hoechst. Image from publication CC-BY-4.0. PMID: 35746834
Immunofluorescence of control and nivalenol (NIV) treated mouse oocytes where α-tubulin is labeled green, centromeres are labeled red and DNA is dyed blue by Hoechst. Image from publication CC-BY-4.0. PMID: 35746834
Immunostaining showing that there is no difference for centromere intensity between mouse WT and Fbxw24-KO germ cells, meanwhile SYCP3 intensity significantly increased in Fbxw24-KO germ cells. DNA in blue, SYCP3 in red, centromere in green. Image from publication CC-BY-4.0. PMID: 35858239
Immunostaining showing that there is no difference for centromere intensity between mouse WT and Fbxw24-KO germ cells, meanwhile SYCP3 intensity significantly increased in Fbxw24-KO germ cells. DNA in blue, SYCP3 in red, centromere in green. Image from publication CC-BY-4.0. PMID: 35858239
Representative maximum z-projections of in situ chromosome spreads from a mouse euploid egg (kinetochores (CREST), green or gray scale) (DNA, blue). Image from publication CC-BY-4.0. PMID: 35810327
Representative maximum z-projections of in situ chromosome spreads from a mouse euploid egg (kinetochores (CREST), green or gray scale) (DNA, blue). Image from publication CC-BY-4.0. PMID: 35810327
Immunofluorescence of HeLa Kyoto cells transiently expressing different Sgo1-GFP constructs (Sgo1-GFP, Sgo1Nmut-GFP, Sgo14A-GFP, or Sgo1Nmut/4A-GFP) and depleted of endogenous Sgo1 using siRNA oligonucleotides showing endogenous Borealin and ACA. DAPI was used for DNA staining. Image from publication CC-BY-4.0. PMID: 35776132
Immunofluorescence of HeLa Kyoto cells transiently expressing different Sgo1-GFP constructs (Sgo1-GFP, Sgo1Nmut-GFP, Sgo14A-GFP, or Sgo1Nmut/4A-GFP) and depleted of endogenous Sgo1 using siRNA oligonucleotides showing endogenous Borealin and ACA. DAPI was used for DNA staining. Image from publication CC-BY-4.0. PMID: 35776132
Uninjected, Control, CBS-KD human oocytes cultured in M16 medium for 8 h to MI for immunofluorescence with Mitotic arrest deficiency 1 (MAD1) (red) and human anti-centromere antibody (CREST) (green). Image from publication CC-BY-4.0. PMID: 36053797
Uninjected, Control, CBS-KD human oocytes cultured in M16 medium for 8 h to MI for immunofluorescence with Mitotic arrest deficiency 1 (MAD1) (red) and human anti-centromere antibody (CREST) (green). Image from publication CC-BY-4.0. PMID: 36053797
Human oocytes in Uninjected, Control, CBS-KD groups were cultured in M16 medium for 8 h to MI and then incubated on ice for 5 min and processed for immunofluorescence with acetylated α-tubulin (red) and CREST antibody (green). Image from publication CC-BY-4.0. PMID: 36053797
Human oocytes in Uninjected, Control, CBS-KD groups were cultured in M16 medium for 8 h to MI and then incubated on ice for 5 min and processed for immunofluorescence with acetylated α-tubulin (red) and CREST antibody (green). Image from publication CC-BY-4.0. PMID: 36053797
Representative image of RPE1 cell with an acentric micronucleus. CREST antibody was used to stain for presence of centromeric proteins. Image from publication CC-BY-4.0. PMID:36266663
Representative image of RPE1 cell with an acentric micronucleus. CREST antibody was used to stain for presence of centromeric proteins. Image from publication CC-BY-4.0. PMID:36266663
Immunofluorescence images of porcine oocytes shortly after NEBD (z-projections, example 1: 14 sections, example 2: 11 sections every 0.19 µm) and after chromosome cluster formation (z-projection, 14 sections every 0.19 µm). F-actin (phalloidin; white); kinetochores (ACA) 1:250; cyan); DNA (Hoechst, magenta,). Image from publication CC-BY-4.0. PMID:36732633
Immunofluorescence images of porcine oocytes shortly after NEBD (z-projections, example 1: 14 sections, example 2: 11 sections every 0.19 µm) and after chromosome cluster formation (z-projection, 14 sections every 0.19 µm). F-actin (phalloidin; white); kinetochores (ACA) 1:250; cyan); DNA (Hoechst, magenta,). Image from publication CC-BY-4.0. PMID:36732633
Control and Pak2-KD metaphase mouse oocytes were labeled with CREST antibody for kinetochores 1:300; purple), anti-tubulin antibody for microtubules (green), and Hoechst 33342 for chromosomes (blue). Image from publication CC-BY-4.0. PMID:36813765
Control and Pak2-KD metaphase mouse oocytes were labeled with CREST antibody for kinetochores 1:300; purple), anti-tubulin antibody for microtubules (green), and Hoechst 33342 for chromosomes (blue). Image from publication CC-BY-4.0. PMID:36813765
Representative images of the morphology of K-M attachments in MI oocytes from both control and BBP-exposed group. α-tubulin (gray); CREST 1:200; red); DNA (blue). Image from publication CC-BY-4.0. PMID:36756972
Representative images of the morphology of K-M attachments in MI oocytes from both control and BBP-exposed group. α-tubulin (gray); CREST 1:200; red); DNA (blue). Image from publication CC-BY-4.0. PMID:36756972
Localization of telomeric sequences in Thylamys elegans and Macropus eugenii. Spread spermatocytes were labeled with antibodies against SYCP3 (blue), centromeric proteins 1:50; red) and telomeric sequences (green) in (D) and (H). Image from publication CC-BY-4.0. PMID:37181752
Localization of telomeric sequences in Thylamys elegans and Macropus eugenii. Spread spermatocytes were labeled with antibodies against SYCP3 (blue), centromeric proteins 1:50; red) and telomeric sequences (green) in (D) and (H). Image from publication CC-BY-4.0. PMID:37181752
Costaining of chromosomes (DAPI, blue) and centromeres 1:250; green) in representative nuclei of metaphase short-term cultures, established from Nf2KO-Setd2KO-Trp53KO-4q9p21 tumor-bearing mice. Image from publication CC-BY-4.0. PMID:37365326
Costaining of chromosomes (DAPI, blue) and centromeres 1:250; green) in representative nuclei of metaphase short-term cultures, established from Nf2KO-Setd2KO-Trp53KO-4q9p21 tumor-bearing mice. Image from publication CC-BY-4.0. PMID:37365326
Representative immunofluorescence images of RioK1 at kinetochores (CREST) 1:50) in prometaphase and metaphase cells. Image from publication CC-BY-4.0. PMID:37263996
Representative immunofluorescence images of RioK1 at kinetochores (CREST) 1:50) in prometaphase and metaphase cells. Image from publication CC-BY-4.0. PMID:37263996
Confocal imaging and quantification of Plk1 signal intensities at centrosomes and kinetochores ) after treating HeLa cells with DMSO or 10 μM of Allopole for 2 h. Arrowheads, centrosomes; arrows, misaligned chromosomes. Signal intensities were quantified from both centrosome signals and randomly chosen three to four kinetochore signals per cell for a total of greater than or equal to 18 cells/experiment. Image from publication CC-BY-4.0. PMID:37603740
Confocal imaging and quantification of Plk1 signal intensities at centrosomes and kinetochores ) after treating HeLa cells with DMSO or 10 μM of Allopole for 2 h. Arrowheads, centrosomes; arrows, misaligned chromosomes. Signal intensities were quantified from both centrosome signals and randomly chosen three to four kinetochore signals per cell for a total of greater than or equal to 18 cells/experiment. Image from publication CC-BY-4.0. PMID:37603740
Pachytene and pachytene-like spermatocytes of A. maximowiczii, MAX (A–C), A. evoronensis “Argi” × A. evoronensis “Evoron”, EVA × EVE (D–F), A. mujanensis × A. maximowiczii, MUJ × MAX (G–I), A. evoronensis “Argi” × A. mujanensis, EVA × MUJ (J–L), and A. evoronensis “Argi” × A. maximowiczii, EVA × MAX (M–O) after immunolocalization of SYCP3, SYCP1, MLH1, γH2A.X, and centromeric proteins (ACA, 1:100). Arrowheads show asynapsed SC regions of the autosomes. CC-BY-4.0. PMID:37263996
Pachytene and pachytene-like spermatocytes of A. maximowiczii, MAX (A–C), A. evoronensis “Argi” × A. evoronensis “Evoron”, EVA × EVE (D–F), A. mujanensis × A. maximowiczii, MUJ × MAX (G–I), A. evoronensis “Argi” × A. mujanensis, EVA × MUJ (J–L), and A. evoronensis “Argi” × A. maximowiczii, EVA × MAX (M–O) after immunolocalization of SYCP3, SYCP1, MLH1, γH2A.X, and centromeric proteins (ACA, 1:100). Arrowheads show asynapsed SC regions of the autosomes. CC-BY-4.0. PMID:37263996
Representative images of chromosome spreading 1:200) of euploid and aneuploid mouse oocytes. Image from publication CC-BY-4.0. PMID:37386516
Representative images of chromosome spreading 1:200) of euploid and aneuploid mouse oocytes. Image from publication CC-BY-4.0. PMID:37386516
RPE-1 cells expressing H2B-GFP and α-Tub-mRFP (magenta) were treated as indicated, fixed and stained for centromeres (ACA) 1:400; white) and DNA (DAPI, blue). BI2536 was added at the IC30 concentration (5 nM). Representative cells are shown, with their measured spindle lengths and centromere dispersion ratios (r). Image from publication CC-BY-4.0. PMID:37639469
RPE-1 cells expressing H2B-GFP and α-Tub-mRFP (magenta) were treated as indicated, fixed and stained for centromeres (ACA) 1:400; white) and DNA (DAPI, blue). BI2536 was added at the IC30 concentration (5 nM). Representative cells are shown, with their measured spindle lengths and centromere dispersion ratios (r). Image from publication CC-BY-4.0. PMID:37639469
RPE1 53BP1‐V5 cells were either left untreated (async = asynchronous) or synchronized in prometaphase in medium containing nocodazole, fixed immediately (M = mitosis) or after 2, 4, or 6 h and co‐stained with V5 and CREST 1:500). Image from publication CC-BY-4.0. PMID:37888778
RPE1 53BP1‐V5 cells were either left untreated (async = asynchronous) or synchronized in prometaphase in medium containing nocodazole, fixed immediately (M = mitosis) or after 2, 4, or 6 h and co‐stained with V5 and CREST 1:500). Image from publication CC-BY-4.0. PMID:37888778
C3a-peptide treatment does not induce chromosome aneuploidy of mouse oocytes. Representative images of MII oocyte immunofluorescence stained with DAPI (blue) and anti-CREST 1:500; magenta) antibodies showing euploidy and aneuploidy in the oocytes, respectively (left). Image from publication CC-BY-4.0. PMID:38066646
C3a-peptide treatment does not induce chromosome aneuploidy of mouse oocytes. Representative images of MII oocyte immunofluorescence stained with DAPI (blue) and anti-CREST 1:500; magenta) antibodies showing euploidy and aneuploidy in the oocytes, respectively (left). Image from publication CC-BY-4.0. PMID:38066646
Pachytene spermatocytes of linnet (A), bullfinch (B), siskin (C), and goldfinch (D) after immunolocalization of SYCP3 (red), MLH1 (green) and centromere proteins (blue). Arrowheads indicate metapolycentromeres and GRC. Image from publication CC-BY-4.0. PMID:38066976
Pachytene spermatocytes of linnet (A), bullfinch (B), siskin (C), and goldfinch (D) after immunolocalization of SYCP3 (red), MLH1 (green) and centromere proteins (blue). Arrowheads indicate metapolycentromeres and GRC. Image from publication CC-BY-4.0. PMID:38066976
Immunostaining with anti-centromere antibody ACA to show centromeres in detached chromosome ends in Zfp541 KO pachytene spermatocytes. Image from publication CC-BY-4.0. PMID:37921559
Immunostaining with anti-centromere antibody ACA to show centromeres in detached chromosome ends in Zfp541 KO pachytene spermatocytes. Image from publication CC-BY-4.0. PMID:37921559

Specifications

Physical State:
Liquid
Storage:
-20°C Short term, -80°C Long term

Release criteria

Protein Concentration:
Lot dependent
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Product code: AHUCNT-1ML

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Unit price$168.50
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Product Overview

Host Species: Human
Clonality: Polyclonal
Species Reactivity: Hamster, Human, Mouse, Rat
RRID: AB_2939058
Purification: Antisera
Physical State: Liquid
Protein Concentration: Lot dependent
Application Notes: Recommended starting dilutions: IHC 1:100; ICC 1:100.
Testing: Serum obtained from an autoimmune patient was tested at a series of dilutions by immunocytochemistry on ethanol-fixed Hep2 cells that were in log-phase growth. The staining pattern obtained was consistent with the pattern expected for anti-centromere staining.
Storage Conditions: -20°C Short term, -80°C Long term
Shipping Conditions: Dry ice
Expiration: Stable for 24 months from receipt.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
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Datasheet

Certificate of Analysis